Skip to main content

Cookies

This site uses cookies to provide you with a more responsive and personalized service. By using this site you agree to the Privacy Policy and Terms of Use. Please read our cookie policy for more information on the use of cookies on this website.

Top
  • COVID-19
    • COVID-19 Resources
  • Solutions
    • By Market
      • Academic Research
      • Agriculture
      • Biobanking
      • Government
      • Pharma and Biotech
      • Research Foundations
    • By Application
      • Clinical Trial Sample Management
      • Discovery, Compound Management and Biologics
      • Molecular Diagnostic Solutions
      • Regenerative Medicine and Cell Therapy
      • Sample Preparation and Lab Services
      • Sample Transport and Cold-Chain Logistics
      • Vaccine Development
    • Geography
      • Asia-Pacific
      • Europe
      • North America
  • Products
    • Azenta Life Sciences Virtual Booth
    • Automated Sample Storage Systems
      • Cold Storage (Ambient to -20°C)
      • Ultra Cold Storage (ULT -80°C)
      • Cryogenic Storage (-190°C)
    • Consumables & Instruments
      • Sample Tube Solutions
      • PCR & Microplate Solutions
      • Sample Cooling & Heating Labware
    • Informatics Platforms & LIMS/LIS
    • Integrations
    • Sterilization Services
    • Microplate Coding Services
  • Services
    • Genomics Services by GENEWIZ
    • Sample and Material Management Solutions
    • Sample and Material Storage
    • Clinical Trial Management
    • Drug Products Storage and Distribution
    • Sample Sourcing
    • Sample Prep and Lab Services
    • Lab Moving and Biological Transport
    • Business Continuity and Risk Mitigation
  • Support
    • Technical Support
    • Product Support Plans
    • Product Updates
    • Sample Tube Pack Request
    • Plate & Seal Sample Request
  • Resources
  • Blog
  • About Us
    • Events
    • About Azenta Life Sciences
    • Management Team
    • Board of Directors
    • Global Locations
    • Investor Relations
    • News
    • Careers
    • Quality
    • Certifications
  • Contact Us

Utility Navigation

  • Life Sciences Customer Login
  • GENEWIZ
  • BioInventory Login
  • Portals Login
  • Portals
  • Careers
Home

Utility Navigation

  • Life Sciences Customer Login
  • GENEWIZ
  • BioInventory Login
  • Portals Login
  • Portals
  • Careers

Main Navigation

  • COVID-19
    Vaccine Development
    Vaccine Development

    Accelerating global operations to manage and process samples critical to vaccine development.

    Azenta Life Sciences Virtual Booth
    Azenta Life Sciences Virtual Booth

    The exhibition experience to your desktop or mobile device

    • COVID-19 Resources
  • Solutions
    • By Market
      • Academic Research
      • Agriculture
      • Biobanking
      • Government
      • Pharma and Biotech
      • Research Foundations
    • By Application
      • Clinical Trial Sample Management
      • Discovery, Compound Management and Biologics
      • Molecular Diagnostic Solutions
      • Regenerative Medicine and Cell Therapy
      • Sample Preparation and Lab Services
      • Sample Transport and Cold-Chain Logistics
      • Vaccine Development
    • Geography
      • Asia-Pacific
      • Europe
      • North America
    Resources Center

    Learn about sample management best practices, gain advice from industry leaders, gain access to global regulatory and logistics guidelines and find simple support tools that can help you make better sample management decisions.

    Resources Center
  • Products
    Azenta Sample Pack and Catalog
    Sample Tube Pack Request

    Improve sample tracking, ensure sample integrity and drive process efficiency with Azenta Sample Storage Tubes. Explore the range, and select the best tube for your workflow.

    Low DNA Binding Microplates
    Low Binding Microplates

    Ultra low DNA binding microplates made from PP, fit for Next Generation Sequencing (NGS) and other sensitive applications with ultra-low DNA input. Off the shelf options. Custom solutions on request.

    • Azenta Life Sciences Virtual Booth
    • Automated Sample Storage Systems
      • Cold Storage (Ambient to -20°C)
      • Ultra Cold Storage (ULT -80°C)
      • Cryogenic Storage (-190°C)
    • Consumables & Instruments
      • Sample Tube Solutions
      • PCR & Microplate Solutions
      • Sample Cooling & Heating Labware
    • Informatics Platforms & LIMS/LIS
    • Integrations
    • Sterilization Services
    • Microplate Coding Services
  • Services
    Clinical Trial Management
    Clinical Trial Management

    Life science organizations must collect and manage large numbers of research samples to bring a new therapeutic or diagnostic to market. This is a complex process.

    Sample Preparation & Lab Analytical Services
    Genomics Services by GENEWIZ

    Comprehensive genomics services provide end to end solutions and best-in-class data quality from the trusted experts in genomics.

    • Genomics Services by GENEWIZ
    • Sample and Material Management Solutions
    • Sample and Material Storage
    • Clinical Trial Management
    • Drug Products Storage and Distribution
    • Sample Sourcing
    • Sample Prep and Lab Services
    • Lab Moving and Biological Transport
    • Business Continuity and Risk Mitigation
  • Support
    disaster recovery
    How Safe Are Your Samples?

    Plastic storage tubes are used in laboratories worldwide to store biological and chemical samples.

    Product Support Plans
    Product Support Plans

    Azenta  Life Sciences' world class service team is committed to ensuring you meet your business objectives by providing a flexible portfolio of service products designed to optimize up-time and productivity.

    • Technical Support
    • Product Support Plans
    • Product Updates
    • Sample Tube Pack Request
    • Plate & Seal Sample Request
  • Resources
  • Blog
  • About Us
    Putting a Sample Kit in an envelope
    Quality Assurance Certifications

    Azenta Life Sciences has established, documented, implemented and currently maintains a quality management system that fulfills the needs of customers.

    About Azenta Life Sciences
    About Azenta Life Sciences

    We are Azenta Life Sciences. We provide unrivaled sample exploration and management solutions to help our customers accelerate discovery, development and delivery.

    • Events
    • About Azenta Life Sciences
    • Management Team
    • Board of Directors
    • Global Locations
    • Investor Relations
    • News
    • Careers
    • Quality
    • Certifications
  • Contact Us
  • 4ti-0970 | FrameStar 96 Well Skirted Optical Bottom PCR Plate | Front

FrameStar® 96 Well Skirted Optical Bottom PCR Plate

Low profile, flat optical bottom, 0.1ml clear polypropylene wells, clear polycarbonate frame, cut corner H1, working volume: <100µl, total well capacity: 180µl

  • Available barcoded on request

Place a Request

Jump to:
  • Overview
  • Key Features
  • Specifications
  • Compatibility
  • Ordering Information
  • Resources
  • Related Products
  • Place a Request

Overview

We have now extended our FrameStar range to include PCR plates with optically clear flat bottom wells for microscopy applications. The FrameStar 96 Well Skirted Optical Bottom PCR Plates (next to their counterpart within our Individual Access range, 4ti-0970/RA) are currently unique in the market, being suitable for use in both microscopy and PCR.

Optical bottom plates are ideal for applications requiring single cell sorting followed by molecular biology techniques such as (q)PCR and sequencing. The flat bottoms enable excellent stackability, making these plates well suited for small sample volume storage such as compound libraries, with no risk of damaging the seal of the plate below. Additionally, the small well volume enables excellent sample recovery.

The low profile wells of this plate are shorter than "standard" profile wells, which decreases the "dead space" between the heated lid of the thermal cycler and the reagents in the well. This reduces condensation forming on the side wall of the tubes, moderating changes in PCR volume and increasing the efficiency of the reaction. This is especially recommended for reaction volumes below 20µl.

The rigid polycarbonate skirt of this design eliminates warping and distortion during PCR, and makes it ideal for use with robotic systems.

Key Features

  • Quality
    • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
    • Stackable
  • Wells
    • Ultra-smooth, uniform, thin-walled polypropylene wells for optimum PCR and real-time (RT-qPCR) results
    • <0.1ml (100µl) working volume, 0.18ml (180µl) total well capacity
  • Frame
    • Rigid polycarbonate frame for added mechanical stability
    • Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
    • Improved seal integrity due to thermal stability of frame
    • Alphanumeric grid reference to aid well and sample identification
  • Use
    • Suitable for microscopy and small volume sample storage
    • Recommended for low volume PCR
    • Compatible with standard multichannel pipettes
    • Ideal for use with robotic systems
  • Options
    • Also available as an Individual Access plate (4ti-0970/RA) with individually detachable and sealable wells
    • Available barcoded upon request; learn more about our barcoding options

Specifications

Parameter FrameStar 96 Well Skirted Optical Bottom PCR Plate
Plate length 127.70 ± 0.25mm
Plate width 85.48 ± 0.25mm
Plate height 16.10 ± 0.25mm
Well depth 12 ± 0.10mm
Well diameter 5.46 ± 0.10mm
Total well capacity 180µl
Working volume <0.1ml (100µl)
Distance to center of A1 from top edge 11.24 ± 0.25mm
Distance to center of A1 from left edge 14.38 ± 0.25mm
Pitch (distance between A1 and A2) 9.00mm

Differences in tube format between the standard conical PCR tube and the flat bottom tubes
Figure 1

Figure 1

Differences in tube format between the standard conical PCR tube and the flat bottom tubes

 

 

 

  • Figure 1 (click on image to expand) illustrates the differences in tube format between the standard conical PCR tube and the flat bottom tubes
  • The surface area of the optical base is 7.74mm2, with a thickness of 0.5mm. The total volume of a conical well is 0.2ml compared to 0.18ml for flat bottom tubes
  • The primary target application for optical bottom plates is assays that require the use of microscopy, for example, work with single cells including fluorescent imaging and cell sorting

Compatibility

  • AGILENT/STRATAGENE
    • Thermal Cyclers (96 well block): Robocycler Gradient
    • qPCR Cyclers (96 well block): AriaMx
  • ANALYTIK JENA/BIOMETRA
    • Thermal Cyclers (96 well block): Flexcycler2 | T1 Thermocycler | Tgradient | Tone | Tadvanced | TProfessional (Standard/Basic) Gradient / XL | Trobot 96 | SpeedCycler2 (SP, SPR)
  • BIO-RAD
    • Thermal Cyclers (96 well block): C1000 Touch | S1000
    • qPCR Cyclers (96 well block): CFX96 Touch | CFX 96 Touch Deep Well | CFX connect
  • BIO-RAD MJ RESEARCH
    • Thermal Cyclers (96 well block): PCT100™/200™/220™/221™/225™/240™
    • qPCR Cyclers (96 well block): Chromo4™ | Opticon2™
  • EPPENDORF
    • Thermal Cyclers (96 well block): MasterCycler® ep/ep gradient/Pro/Pro S/nexus/nexus gradient/nexus SX1/nexus GSX1
    • qPCR Cyclers (96 well block): MasterCycler™ ep realplex
  • GE HEALTHCARE/AMERSHAM
    • Sequencers (96 well block): MegaBACE™ 500 | MegaBACE™ 1000 mark 2
  • PEQLAB/VWR
    • Thermal Cyclers (96 well block): peqSTAR 96X
  • SENSOQUEST
    • Thermal Cyclers (96 well block): Labcycler
  • TECHNE
    • Thermal Cyclers (96 well block): TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene
    • qPCR Cyclers (96 well block): Quantica

For recommended plate options depending on manufacturer, block type, and instrument please refer to the Azenta Life Sciences Plate Instrument Compatibility Table and look for the FrameStar® 96 Well Skirted PCR Plate (4ti-0960).

Ordering Information

Use these part numbers to request a quote, a sample or to contact an expert:

Part number Description
FrameStar 96 Well Skirted Optical Bottom PCR Plate
Low profile, flat optical bottom, 0.1ml clear polypropylene wells, clear polycarbonate frame, cut corner H1, working volume: <100µl, total well capacity: 180µl
4ti-0970 clear wells, clear frame; 50 plates

Resources

Consumable Effects on Low Volume TaqMan® Assays for Quantitative PCR on Roche LightCycler® 480

Consumable Effects on Low Volume TaqMan® Assays for Quantitative PCR on Roche LightCycler® 480

Application Notes & Tech Notes
Miniaturization of a qPCR Assay with Reduced Reagent Volumes to Achieve Cost Savings in Gene Expression Analyses in Rumen Epithelium

Miniaturization of a qPCR Assay with Reduced Reagent Volumes to Achieve Cost Savings in Gene Expression Analyses in Rumen Epithelium

Application Notes & Tech Notes
Azenta Life Sciences Sample Consumables and Instruments Catalog

Azenta Life Sciences Sample Consumables and Instruments Catalog

Catalogs
PCR Plate-Instrument Compatibility

PCR Plate-Instrument Compatibility

Brochures & Sell Sheets
FrameStar® 96 Well Skirted Optical Bottom PCR Plate Technical Drawing

FrameStar® 96 Well Skirted Optical Bottom PCR Plate Technical Drawing

Technical Drawings
Horizontal Scroll
On

Related Products

Similar Products

See below for a selection of products in the same range:

4ti-0960 | FrameStar 96 Well Skirted PCR Plate | Front

FrameStar® 96 Well Skirted PCR Plate

BUY ONLINE
On

Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H1; working volume: <100µl, total well capacity: 200µl

4ti-0970/RA | Individual Access 96 Well Skirted Optical Bottom PCR Plate | Bottom Detail

Individual Access 96 Well Skirted Optical Bottom PCR Plate

Off

96 individual tubes in plate format, low profile, flat optical bottom, 0.1ml clear polypropylene tubes, black rigid polycarbonate frame, cut corner H1, working volume: <100µl, total well capacit

4ti-0975/RA | Individual Access 96 Well Skirted Flat Bottom PCR Plate, 2D Coded | Bottom Detail

Individual Access 96 Well Skirted Flat Bottom PCR Plate, 2D Coded

Off

96 individual tubes in plate format, low profile, flat bottom 2D coded, 0.1ml clear polypropylene tubes, white rigid polycarbonate frame, linear barcoded, cut corner H1, working volume: <100µl,

Horizontal Scroll
On

Compatible Products

See below for a selection of compatible products:

4ti-0665 | Automated Roll Heat Sealer (formerly a4S)

Automated Roll Heat Sealer (formerly a4S)

BUY ONLINE
On

The perfect solution for automated heat sealing of microplates, PCR plates, assay plates and storage plates used in medium to high-throughput or full automation applications

59-2000 | Semi-Automated Sheet Heat Sealer (formerly IntelliXseal SA) | Side | Holder & Plate Detail

Semi-Automated Sheet Heat Sealer (formerly IntelliXseal™ SA)

BUY ONLINE
On

Semi-automated sheet heat sealer compatible with a wide range of seals and microplates, PCR and assay plates, as well as storage plates of differing designs and heights

4ti-05381/RA | Individual Access Pierce Heat Seal Strong

Individual Access Pierce Heat Seal Strong

Off

96 individual foil seal spots on a removable backing, for sealing of individual wells; suitable for storage or PCR

4ti-0565 | Optically Clear Windowed qPCR Seal | Peeling Detail

Optically Clear Windowed qPCR Seal

Off

Adhesive film with 96 optically clear windows, peelable; suitable for qPCR and optical applications

4ti-0531 | Pierce Heat Seal | Piercing Detail

Pierce Heat Seal

BUY ONLINE
On

Pierceable heat sealing foil, high solvent resistance, resealable, suitable for PCR/storage/shipping

Horizontal Scroll
On

Place a Request

Request a Quote, Free Samples, or an Expert Consultation

Want to learn more about this product? Speak to one of our experts, or request a quote or free samples by filling out and submitting the form.

Home
Facebook twitter youtube linkedin
Copyright © 2023 Azenta US, Inc.

Footer menu

  • Privacy Policy
  • Cookie Policy
  • Terms and Conditions
  • Terms of Use
  • Careers
  • Subscribe