4ti-0960 | FrameStar 96 Well Skirted PCR Plate | Front
4ti-0960 | FrameStar 96 Well Skirted PCR Plate
4ti-0960 | FrameStar 96 Well Skirted PCR Plate | Corner Detail
4ti-0960/B | FrameStar 96 Well Skirted PCR Plate | Front
4ti-0960/B | FrameStar 96 Well Skirted PCR Plate
4ti-0960/B | FrameStar 96 Well Skirted PCR Plate | Corner Detail
4ti-0960/C | FrameStar 96 Well Skirted PCR Plate | Front
4ti-0960/C | FrameStar 96 Well Skirted PCR Plate
4ti-0960/G | FrameStar 96 Well Skirted PCR Plate | Front
4ti-0960/G | FrameStar 96 Well Skirted PCR Plate
4ti-0960/G | FrameStar 96 Well Skirted PCR Plate | Corner Detail
4ti-0960/R | FrameStar 96 Well Skirted PCR Plate | Front
4ti-0960/R | FrameStar 96 Well Skirted PCR Plate
4ti-0960/R | FrameStar 96 Well Skirted PCR Plate | Corner Detail
4ti-0960/W | FrameStar 96 Well Skirted PCR Plate | Front
4ti-0960/W | FrameStar 96 Well Skirted PCR Plate
4ti-0960/W | FrameStar 96 Well Skirted PCR Plate | Corner Detail
4ti-0960/X | FrameStar 96 Well Skirted PCR Plate | Front
4ti-0960/X | FrameStar 96 Well Skirted PCR Plate
4ti-0960/X | FrameStar 96 Well Skirted PCR Plate | Corner Detail
4ti-0960/RIG | FrameStar 96 Well Skirted PCR Plate | Front
4ti-0960/RIG | FrameStar 96 Well Skirted PCR Plate
4ti-0960/RIG | FrameStar 96 Well Skirted PCR Plate | Corner Detail
4ti-0961 | FrameStar 96 Well Skirted PCR Plate | Front
4ti-0961 | FrameStar 96 Well Skirted PCR Plate
4ti-0961 | FrameStar 96 Well Skirted PCR Plate | Corner Detail
4ti-0961/C | FrameStar 96 Well Skirted PCR Plate | Front
4ti-0966 | FrameStar 96 Well Skirted PCR Plate | Front
4ti-0966 | FrameStar 96 Well Skirted PCR Plate

FrameStar® 96 Well Skirted PCR Plate

Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H1; working volume: <100µl, total well capacity: 200µl

Available barcoded on request
Ultra-low DNA binding option available

BUY ONLINE

BUY ONLINE
Online availability to US only

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Overview
Low DNA Binding Products
Key Features
Specifications
Compatibility
Ordering Information
Resources
Related Products

Overview

The low profile wells of this plate are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well. This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction.

This is especially recommended for reaction volumes below 20µl. The rigid polycarbonate skirt of this design eliminates warping and distortion during PCR, and makes it ideal for use with robotic systems.

Low DNA Binding Products

Azenta Life Sciences offer low binding products, recommended to support sensitive techniques such as qPCR and Next Generation Sequencing (NGS), as well as storage of samples.
Manufactured using selected low-bind polymers, DNA loss is minimized during incubation and sample transfer, making it the ideal solution for use in applications with low DNA input.
Low binding feature results from selected low-bind polymers, no coating is used to achieve the binding characteristics. No contamination or modification of the samples.
Tested under a broad temperature range. Maximum DNA recovery after low temperature storage and high temperature incubations.
No detectable DNA loss during incubation steps and sample transfer in NGS sample prep and library construction. Ideally suited for sensitive applications with ultra-low nucleic acid input.

Key Features

Quality
- Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
- Stackable
Wells
- Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
- <0.1ml (100µl) working volume, 0.2ml (200µl) total well capacity
Frame
- Rigid polycarbonate frame for added mechanical stability
- Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
- Improved seal integrity due to thermal stability of frame
- Alphanumeric grid reference to aid well and sample identification
Use
- Recommended for low volume PCR
- Ideal for use with robotic systems
- Compatible with standard multichannel pipettes
Options
- Available with the following frame color options for the clear well variety: purple, blue, clear, green, red, black, and white
- Also available with a black frame and with a clear frame for the white well variety, for optimum signal-to-noise ratio when using fluorescent based assays
- Also available as a black frame with black wells for non-PCR fluorescent applications
- Extra rigid skirt option (4ti-0960/RIG) for use with Perkin-Elmer^®Sciclone, Beckman, Hamilton, and other automation systems: eliminates the robotic grip picking up more than one plate at a time
- Ethylene oxide treated option available (4ti-OX960C/SBC) for forensic use; learn more about our ethylene oxide treated range
- Ultra-low DNA binding option available (4ti-LB0960/RIG) for sensitive applications with ultra-low DNA input and for maximum DNA recovery after low temperature storage and high temperature incubation; learn more about our low binding range
- Available barcoded upon request; learn more about our barcoding options
- Combi packs available (for 4ti-0960) with Optically Clear Windowed qPCR Seal (4ti-0565)
- Clear Polystyrene Lid (4ti-0287) available for 4ti-0960/RIG

Specifications

Parameter	FrameStar 96 Well Skirted PCR Plate
Plate length	127.76 ± 0.25mm
Plate width	85.48 ± 0.25mm
Plate height	16.10 ± 0.25mm
Well depth	15.10 ± 0.10mm
Well diameter	5.50 ± 0.10mm
Total well capacity	0.2ml (200µl)
Working volume	<0.1ml (100µl)
Distance to center of A1 from top edge	11.24 ± 0.25mm
Distance to center of A1 from left edge	14.38 ± 0.25mm
Pitch (distance between A1 and A2)	9.00mm

Compatibility

AGILENT/STRATAGENE
- Thermal Cyclers (96 well block): Robocycler Gradient
- qPCR Cyclers (96 well block): AriaMx
ANALYTIK JENA/BIOMETRA
- Thermal Cyclers (96 well block): Flexcycler2 | T1 Thermocycler | Tgradient | Tone | Tadvanced | TProfessional (Standard/Basic) Gradient/XL | Trobot 96 | SpeedCycler2 (SP, SPR)
BIO-RAD
- Thermal Cyclers (96 well block): C1000 Touch | S1000
- qPCR Cyclers (96 well block): CFX96 Touch | CFX 96 Touch Deep Well | CFX connect
BIO-RAD MJ RESEARCH
- Thermal Cyclers (96 well block): PCT100™/200™/220™/221™/225™/240™
- qPCR Cyclers (96 well block): Chromo4™ | Opticon2™
EPPENDORF
- Thermal Cyclers (96 well block): MasterCycler^® ep/ep gradient/Pro/Pro S/nexus/nexus gradient/nexus SX1/nexus GSX1
- qPCR Cyclers (96 well block): MasterCycler™ ep realplex
GE HEALTHCARE/AMERSHAM
- Sequencers (96 well block): MegaBACE™ 500 | MegaBACE™ 1000 mark 2
PEQLAB/VWR
- Thermal Cyclers (96 well block): peqSTAR 96X
SENSOQUEST
- Thermal Cyclers (96 well block): Labcycler
TECHNE
- Thermal Cyclers (96 well block): TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene
- qPCR Cyclers (96 well block): Quantica

For recommended plate options depending on manufacturer, block type, and instrument please refer to the Azenta Life Sciences Plate Instrument Compatibility Table.

Ordering Information

Use these part numbers to request a quote, a sample or to contact an expert:

Part number	Description
FrameStar 96 Well Skirted PCR Plate Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H1; working volume: <100µl, total well capacity: 200µl
4ti-0960	clear wells, purple frame; 50 plates
4ti-0960/B	clear wells, blue frame; 50 plates
4ti-0960/C BUY ONLINE*	clear wells, clear frame; 50 plates
4ti-0960/G	clear wells, green frame; 50 plates
4ti-0960/R	clear wells, red frame; 50 plates
4ti-0960/X	clear wells, black frame; 50 plates
4ti-0960/W BUY ONLINE*	clear wells, white frame; 50 plates
4ti-0961	white wells, black frame; 50 plates
4ti-0961/C	white wells, clear frame; 50 plates
4ti-0966	black wells, black frame; 50 plates
4ti-OX960C/SBC	4ti-0960/C for forensic use, ethylene oxide treated and single barcoded; 20 plates
4ti-0960/RIG	4ti-0960/X with extra rigid skirt; 50 plates
4ti-LB0960/RIG	4ti-0960/RIG, with low DNA binding properties; 50 plates
Combi packs
4ti-0960/0565	4ti-0960 + Optically Clear Windowed qPCR Seal (4ti-0565); 50 plates + 50 seals
Lids
4ti-0287	FrameStar 96 NGS Lid (4ti-0287), for use with 4ti-0960/RIG; 50 lids

*Online availability to US only

Resources

Consumable Effects on Low Volume TaqMan® Assays for Quantitative PCR on Roche LightCycler® 480

Application Notes & Tech Notes

Miniaturization of a qPCR Assay with Reduced Reagent Volumes to Achieve Cost Savings in Gene Expression Analyses in Rumen Epithelium

Application Notes & Tech Notes

Low DNA Binding Products Brochure

Brochures & Sell Sheets

PCR Plate-Instrument Compatibility

Brochures & Sell Sheets

Azenta Life Sciences Sample Consumables and Instruments Catalog

Catalogs

Avoiding DNA Adsorption to Tube Walls in NGS Sample Preparation

Publications & Posters