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  • 4ti-0960 | FrameStar 96 Well Skirted PCR Plate | Front
  • 4ti-0960 | FrameStar 96 Well Skirted PCR Plate
  • 4ti-0960 | FrameStar 96 Well Skirted PCR Plate | Corner Detail
  • 4ti-0960/B | FrameStar 96 Well Skirted PCR Plate | Front
  • 4ti-0960/B | FrameStar 96 Well Skirted PCR Plate
  • 4ti-0960/B | FrameStar 96 Well Skirted PCR Plate | Corner Detail
  • 4ti-0960/C | FrameStar 96 Well Skirted PCR Plate | Front
  • 4ti-0960/C | FrameStar 96 Well Skirted PCR Plate
  • 4ti-0960/G | FrameStar 96 Well Skirted PCR Plate | Front
  • 4ti-0960/G | FrameStar 96 Well Skirted PCR Plate
  • 4ti-0960/G | FrameStar 96 Well Skirted PCR Plate | Corner Detail
  • 4ti-0960/R | FrameStar 96 Well Skirted PCR Plate | Front
  • 4ti-0960/R | FrameStar 96 Well Skirted PCR Plate
  • 4ti-0960/R | FrameStar 96 Well Skirted PCR Plate | Corner Detail
  • 4ti-0960/W | FrameStar 96 Well Skirted PCR Plate | Front
  • 4ti-0960/W | FrameStar 96 Well Skirted PCR Plate
  • 4ti-0960/W | FrameStar 96 Well Skirted PCR Plate | Corner Detail
  • 4ti-0960/X | FrameStar 96 Well Skirted PCR Plate | Front
  • 4ti-0960/X | FrameStar 96 Well Skirted PCR Plate
  • 4ti-0960/X | FrameStar 96 Well Skirted PCR Plate | Corner Detail
  • 4ti-0960/RIG | FrameStar 96 Well Skirted PCR Plate | Front
  • 4ti-0960/RIG | FrameStar 96 Well Skirted PCR Plate
  • 4ti-0960/RIG | FrameStar 96 Well Skirted PCR Plate | Corner Detail
  • 4ti-0961 | FrameStar 96 Well Skirted PCR Plate | Front
  • 4ti-0961 | FrameStar 96 Well Skirted PCR Plate
  • 4ti-0961 | FrameStar 96 Well Skirted PCR Plate | Corner Detail
  • 4ti-0961/C | FrameStar 96 Well Skirted PCR Plate | Front
  • 4ti-0966 | FrameStar 96 Well Skirted PCR Plate | Front
  • 4ti-0966 | FrameStar 96 Well Skirted PCR Plate

FrameStar® 96 Well Skirted PCR Plate

Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H1; working volume: <100µl, total well capacity: 200µl

  • Available barcoded on request
  • Ultra-low DNA binding option available

Place a Request

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Online availability to US only

Jump to:
  • Overview
  • Low DNA Binding Products
  • Key Features
  • Specifications
  • Compatibility
  • Ordering Information
  • Resources
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  • Place a Request

Overview

The low profile wells of this plate are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well. This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction.

This is especially recommended for reaction volumes below 20µl. The rigid polycarbonate skirt of this design eliminates warping and distortion during PCR, and makes it ideal for use with robotic systems.

Low DNA Binding Products

  • Azenta Life Sciences offer low binding products, recommended to support sensitive techniques such as qPCR and Next Generation Sequencing (NGS), as well as storage of samples.
  • Manufactured using selected low-bind polymers, DNA loss is minimized during incubation and sample transfer, making it the ideal solution for use in applications with low DNA input.
  • Low binding feature results from selected low-bind polymers, no coating is used to achieve the binding characteristics. No contamination or modification of the samples.
  • Tested under a broad temperature range. Maximum DNA recovery after low temperature storage and high temperature incubations.
  • No detectable DNA loss during incubation steps and sample transfer in NGS sample prep and library construction. Ideally suited for sensitive applications with ultra-low nucleic acid input.

Key Features

  • Quality
    • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
    • Stackable
  • Wells
    • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
    • <0.1ml (100µl) working volume, 0.2ml (200µl) total well capacity
  • Frame
    • Rigid polycarbonate frame for added mechanical stability
    • Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
    • Improved seal integrity due to thermal stability of frame
    • Alphanumeric grid reference to aid well and sample identification
  • Use
    • Recommended for low volume PCR
    • Ideal for use with robotic systems
    • Compatible with standard multichannel pipettes
  • Options
    • Available with the following frame color options for the clear well variety: purple, blue, clear, green, red, black, and white
    • Also available with a black frame and with a clear frame for the white well variety, for optimum signal-to-noise ratio when using fluorescent based assays
    • Also available as a black frame with black wells for non-PCR fluorescent applications
    • Extra rigid skirt option (4ti-0960/RIG) for use with Perkin-Elmer®Sciclone, Beckman, Hamilton, and other automation systems: eliminates the robotic grip picking up more than one plate at a time
    • Ethylene oxide treated option available (4ti-OX960C/SBC) for forensic use; learn more about our ethylene oxide treated range 
    • Ultra-low DNA binding option available (4ti-LB0960/RIG) for sensitive applications with ultra-low DNA input and for maximum DNA recovery after low temperature storage and high temperature incubation; learn more about our low binding range
    • Available barcoded upon request; learn more about our barcoding options
    • Combi packs available (for 4ti-0960) with Optically Clear Windowed qPCR Seal (4ti-0565)
    • Clear Polystyrene Lid (4ti-0287) available for 4ti-0960/RIG

Specifications

Parameter FrameStar 96 Well Skirted PCR Plate
Plate length 127.76 ± 0.25mm
Plate width 85.48 ± 0.25mm
Plate height 16.10 ± 0.25mm
Well depth 15.10 ± 0.10mm
Well diameter 5.50 ± 0.10mm
Total well capacity 0.2ml (200µl)
Working volume <0.1ml (100µl)
Distance to center of A1 from top edge 11.24 ± 0.25mm
Distance to center of A1 from left edge 14.38 ± 0.25mm
Pitch (distance between A1 and A2) 9.00mm

Compatibility

  • AGILENT/STRATAGENE
    • Thermal Cyclers (96 well block): Robocycler Gradient
    • qPCR Cyclers (96 well block): AriaMx
  • ANALYTIK JENA/BIOMETRA
    • Thermal Cyclers (96 well block): Flexcycler2 | T1 Thermocycler | Tgradient | Tone | Tadvanced | TProfessional (Standard/Basic) Gradient/XL | Trobot 96 | SpeedCycler2 (SP, SPR)
  • BIO-RAD
    • Thermal Cyclers (96 well block): C1000 Touch | S1000
    • qPCR Cyclers (96 well block): CFX96 Touch | CFX 96 Touch Deep Well | CFX connect
  • BIO-RAD MJ RESEARCH
    • Thermal Cyclers (96 well block): PCT100™/200™/220™/221™/225™/240™
    • qPCR Cyclers (96 well block): Chromo4™ | Opticon2™
  • EPPENDORF
    • Thermal Cyclers (96 well block): MasterCycler® ep/ep gradient/Pro/Pro S/nexus/nexus gradient/nexus SX1/nexus GSX1
    • qPCR Cyclers (96 well block): MasterCycler™ ep realplex
  • GE HEALTHCARE/AMERSHAM
    • Sequencers (96 well block): MegaBACE™ 500 | MegaBACE™ 1000 mark 2
  • PEQLAB/VWR
    • Thermal Cyclers (96 well block): peqSTAR 96X
  • SENSOQUEST
    • Thermal Cyclers (96 well block): Labcycler
  • TECHNE
    • Thermal Cyclers (96 well block): TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene
    • qPCR Cyclers (96 well block): Quantica

For recommended plate options depending on manufacturer, block type, and instrument please refer to the Azenta Life Sciences Plate Instrument Compatibility Table.

Ordering Information

Use these part numbers to request a quote, a sample or to contact an expert:

Part number Description
FrameStar 96 Well Skirted PCR Plate
Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H1; working volume: <100µl, total well capacity: 200µl
4ti-0960 clear wells, purple frame; 50 plates
4ti-0960/B clear wells, blue frame; 50 plates
4ti-0960/C
BUY ONLINE*
clear wells, clear frame; 50 plates
4ti-0960/G clear wells, green frame; 50 plates
4ti-0960/R clear wells, red frame; 50 plates
4ti-0960/X clear wells, black frame; 50 plates
4ti-0960/W
BUY ONLINE*
clear wells, white frame; 50 plates
4ti-0961 white wells, black frame; 50 plates
4ti-0961/C white wells, clear frame; 50 plates
4ti-0966 black wells, black frame; 50 plates
4ti-OX960C/SBC 4ti-0960/C for forensic use, ethylene oxide treated and single barcoded; 20 plates
4ti-0960/RIG 4ti-0960/X with extra rigid skirt; 50 plates
4ti-LB0960/RIG 4ti-0960/RIG, with low DNA binding properties; 50 plates
Combi packs
4ti-0960/0565 4ti-0960 + Optically Clear Windowed qPCR Seal (4ti-0565); 50 plates + 50 seals
Lids
4ti-0287 FrameStar 96 NGS Lid (4ti-0287), for use with 4ti-0960/RIG; 50 lids

*Online availability to US only

Resources

Consumable Effects on Low Volume TaqMan® Assays for Quantitative PCR on Roche LightCycler® 480

Consumable Effects on Low Volume TaqMan® Assays for Quantitative PCR on Roche LightCycler® 480

Application Notes & Tech Notes
Miniaturization of a qPCR Assay with Reduced Reagent Volumes to Achieve Cost Savings in Gene Expression Analyses in Rumen Epithelium

Miniaturization of a qPCR Assay with Reduced Reagent Volumes to Achieve Cost Savings in Gene Expression Analyses in Rumen Epithelium

Application Notes & Tech Notes
Low DNA Binding Products Brochure

Low DNA Binding Products Brochure

Brochures & Sell Sheets
PCR Plate-Instrument Compatibility

PCR Plate-Instrument Compatibility

Brochures & Sell Sheets
Azenta Life Sciences Sample Consumables and Instruments Catalog

Azenta Life Sciences Sample Consumables and Instruments Catalog

Catalogs
Avoiding DNA Adsorption to Tube Walls in NGS Sample Preparation

Avoiding DNA Adsorption to Tube Walls in NGS Sample Preparation

Publications & Posters
FrameStar® 96 Well Skirted PCR Plate Technical Drawing

FrameStar® 96 Well Skirted PCR Plate Technical Drawing

Technical Drawings
FrameStar® 96 Well Skirted PCR Plate, Extra Rigid Technical Drawing

FrameStar® 96 Well Skirted PCR Plate, Extra Rigid Technical Drawing

Technical Drawings
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Related Products

Similar Products

See below for a selection of products in the same range:

4ti-0950/C | FrameStar 96 Well Semi-Skirted PCR Plate, Roche Style | Front

FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style

BUY ONLINE
On

Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H12; working volume: <100µl, total well capacity: 200µl; designed for use on Roche LightCycler® 96 and 480

4ti-0770/R | FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style | Front

FrameStar® 96 Well Semi-Skirted PCR Plate, ABI® Style

BUY ONLINE
On

Standard profile, 0.2ml polypropylene wells, polycarbonate frame, cut corner A12; working volume: <200µl, total well capacity: 300µl

4ti-0740 | 96 Well Skirted PCR Plate | Front

96 Well Skirted PCR Plate

BUY ONLINE
On

Low profile, 0.1ml wells, polypropylene, cut corner H1, working volume: <100µl, total well capacity: 200µl; universal 96 well skirted plate, designed for use on standard thermal cyclers

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On

Compatible Products

See below for a selection of compatible products:

4ti-0665 | Automated Roll Heat Sealer (formerly a4S)

Automated Roll Heat Sealer (formerly a4S)

BUY ONLINE
On

The perfect solution for automated heat sealing of microplates, PCR plates, assay plates and storage plates used in medium to high-throughput or full automation applications

59-2000 | Semi-Automated Sheet Heat Sealer (formerly IntelliXseal SA) | Side | Holder & Plate Detail

Semi-Automated Sheet Heat Sealer (formerly IntelliXseal™ SA)

BUY ONLINE
On

Semi-automated sheet heat sealer compatible with a wide range of seals and microplates, PCR and assay plates, as well as storage plates of differing designs and heights

4ti-0565 | Optically Clear Windowed qPCR Seal | Peeling Detail

Optically Clear Windowed qPCR Seal

Off

Adhesive film with 96 optically clear windows, peelable; suitable for qPCR and optical applications

4ti-0531 | Pierce Heat Seal | Piercing Detail

Pierce Heat Seal

BUY ONLINE
On

Pierceable heat sealing foil, high solvent resistance, resealable, suitable for PCR/storage/shipping

PCR Plate Lids | Group

PCR Plate Lid

Off

Rigid polystyrene lids for PCR, designed to give a quick and easy sealing solution to protect samples from contamination and evaporation

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On

Place a Request

Request a Quote, Free Samples, or an Expert Consultation

Want to learn more about this product? Speak to one of our experts, or request a quote or free samples by filling out and submitting the form.

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