FrameStar® 96 Well Skirted PCR Plate
Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H1; working volume: <100µl, total well capacity: 200µl
- Available barcoded on request
BUY NOW (US ONLY)
Overview
The low profile wells of this plate are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well. This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction.
This is especially recommended for reaction volumes below 20µl. The rigid polycarbonate skirt of this design eliminates warping and distortion during PCR, and makes it ideal for use with robotic systems.
Key Features
-
Quality
- Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
- Stackable
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Wells
- Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
- <0.1ml (100µl) working volume, 0.2ml (200µl) total well capacity
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Frame
- Rigid polycarbonate frame for added mechanical stability
- Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
- Improved seal integrity due to thermal stability of frame
- Alphanumeric grid reference to aid well and sample identification
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Use
- Recommended for low volume PCR
- Ideal for use with robotic systems
- Compatible with standard multichannel pipettes
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Options
- Available with the following frame color options for the clear well variety: purple, blue, clear, green, red, black, and white
- Also available with a black frame and with a clear frame for the white well variety, for optimum signal-to-noise ratio when using fluorescent based assays
- Also available as a black frame with black wells for non-PCR fluorescent applications
- Extra rigid skirt option (4ti-0960/RIG) for use with Perkin-Elmer®Sciclone, Beckman, Hamilton, and other automation systems: eliminates the robotic grip picking up more than one plate at a time
- Ethylene oxide treated option available (4ti-OX960C/SBC) for forensic use; learn more about our ethylene oxide treated range
- Ultra-low DNA binding option available (4ti-LB0960/RIG) for sensitive applications with ultra-low DNA input and for maximum DNA recovery after low temperature storage and high temperature incubation; learn more about our low binding range
- Available barcoded upon request; learn more about our barcoding options
- Combi packs available (for 4ti-0960) with Optically Clear Windowed qPCR Seal (4ti-0565)
- Clear Polystyrene Lid (4ti-0287) available for 4ti-0960/RIG
Specifications
| Parameter | FrameStar 96 Well Skirted PCR Plate |
| Plate length | 127.76 ± 0.25mm |
| Plate width | 85.48 ± 0.25mm |
| Plate height | 16.10 ± 0.25mm |
| Well depth | 15.10 ± 0.10mm |
| Well diameter | 5.50 ± 0.10mm |
| Total well capacity | 0.2ml (200µl) |
| Working volume | <0.1ml (100µl) |
| Distance to center of A1 from top edge | 11.24 ± 0.25mm |
| Distance to center of A1 from left edge | 14.38 ± 0.25mm |
| Pitch (distance between A1 and A2) | 9.00mm |
Compatibility
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AGILENT/STRATAGENE
- Thermal Cyclers (96 well block): Robocycler Gradient
- qPCR Cyclers (96 well block): AriaMx
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ANALYTIK JENA/BIOMETRA
- Thermal Cyclers (96 well block): Flexcycler2 | T1 Thermocycler | Tgradient | Tone | Tadvanced | TProfessional (Standard/Basic) Gradient/XL | Trobot 96 | SpeedCycler2 (SP, SPR)
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BIO-RAD
- Thermal Cyclers (96 well block): C1000 Touch | S1000
- qPCR Cyclers (96 well block): CFX96 Touch | CFX 96 Touch Deep Well | CFX connect
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BIO-RAD MJ RESEARCH
- Thermal Cyclers (96 well block): PCT100™/200™/220™/221™/225™/240™
- qPCR Cyclers (96 well block): Chromo4™ | Opticon2™
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EPPENDORF
- Thermal Cyclers (96 well block): MasterCycler® ep/ep gradient/Pro/Pro S/nexus/nexus gradient/nexus SX1/nexus GSX1
- qPCR Cyclers (96 well block): MasterCycler™ ep realplex
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GE HEALTHCARE/AMERSHAM
- Sequencers (96 well block): MegaBACE™ 500 | MegaBACE™ 1000 mark 2
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PEQLAB/VWR
- Thermal Cyclers (96 well block): peqSTAR 96X
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SENSOQUEST
- Thermal Cyclers (96 well block): Labcycler
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TECHNE
- Thermal Cyclers (96 well block): TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene
- qPCR Cyclers (96 well block): Quantica
For recommended plate options depending on manufacturer, block type, and instrument please refer to the Azenta Life Sciences Plate Instrument Compatibility Table.
Ordering Information
Use these part numbers to request a quote, a sample or to contact an expert:
| Part number | Description |
| FrameStar 96 Well Skirted PCR Plate Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H1; working volume: <100µl, total well capacity: 200µl |
|
| 4ti-0960 | clear wells, purple frame; 50 plates |
| 4ti-0960/B | clear wells, blue frame; 50 plates |
| 4ti-0960/C BUY NOW (US ONLY) |
clear wells, clear frame; 50 plates |
| 4ti-0960/G | clear wells, green frame; 50 plates |
| 4ti-0960/R | clear wells, red frame; 50 plates |
| 4ti-0960/X | clear wells, black frame; 50 plates |
| 4ti-0960/W | clear wells, white frame; 50 plates |
| 4ti-0961 | white wells, black frame; 50 plates |
| 4ti-0961/C | white wells, clear frame; 50 plates |
| 4ti-0966 | black wells, black frame; 50 plates |
| 4ti-OX960C/SBC | 4ti-0960/C for forensic use, ethylene oxide treated and single barcoded; 20 plates |
| 4ti-0960/RIG | 4ti-0960/X with extra rigid skirt; 50 plates |
| 4ti-LB0960/RIG | 4ti-0960/RIG, with low DNA binding properties; 50 plates |
| Combi packs | |
| 4ti-0960/0565 | 4ti-0960 + Optically Clear Windowed qPCR Seal (4ti-0565); 50 plates + 50 seals |
| Lids | |
| 4ti-0287 | FrameStar 96 NGS Lid (4ti-0287), for use with 4ti-0960/RIG; 50 lids |
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