4ti-0730 | FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style | Front
4ti-0730 | FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style
4ti-0730 | FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style | Corner Detail
4ti-0730/B | FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style | Front
4ti-0730/B | FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style
4ti-0730/B | FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style | Corner Detail
4ti-0730/C | FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style | Front
4ti-0730/C | FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style
4ti-0730/C | FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style | Corner Detail
4ti-0730/G | FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style | Front
4ti-0730/G | FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style
4ti-0730/G | FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style | Corner Detail
4ti-0730/R | FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style | Front
4ti-0730/R | FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style
4ti-0730/R | FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style | Corner Detail
4ti-0730/X | FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style | Front
4ti-0730/X | FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style
4ti-0730/X | FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style | Corner Detail
4ti-0731 | FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style | Front
4ti-0731 | FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style

FrameStar® 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style

Standard profile, 0.2ml polypropylene wells, polycarbonate frame with upstand, cut corner A12, working volume: <200µl, total well capacity: 300µl; designed for use on ABI^® instruments

Available barcoded on request

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Overview
Key Features
Specifications
Compatibility
Ordering Information
Resources
Related Products

Overview

This ABI^® Style PCR plate offers the benefits of our 2-component design to ABI^® standard block users. This design combines the advantages of ultra-thin wall polypropylene tubes for optimum PCR results with a rigid polycarbonate skirt and deck for the highest thermal stability. Learn more about the features and benefits of the FrameStar range.

We recommend this semi-skirted plate for use with ABI^® thermal cyclers and sequencers; it can be used directly with ABI^® instruments with no adapters necessary. The only case where this is not true is with the ABI Fast Block thermal cyclers; in this case, using our FrameStar Fast Plate is recommended instead.

Key Features

Quality
- Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
Wells
- Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time quantitative PCR (RT-qPCR) results
- <0.2ml (200µl) working volume, 0.3ml (300µl) total well capacity
Frame
- Rigid polycarbonate frame for added mechanical stability
- Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
- Improved seal integrity due to thermal stability of frame
- Alphanumeric grid reference to aid well and sample identification
Use
- Ideal for use with ABI^® thermal cyclers & sequencers
- Recommended for low volume PCR
- Compatible with standard multichannel pipettes
- Ideal for use with robotic systems
Options
- Available with the following frame color options for the clear well variety: purple, blue, clear, green, red, and black
- Ethylene oxide treated option available for forensic use; learn more about our ethylene oxide treated range
- Available barcoded upon request; learn more about our barcoding options
- Combi packs available (for 4ti-0730/C) with Optically Clear Windowed qPCR Seal (4ti-0565)

Specifications

Parameter	FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style
Plate length	124.26 ± 0.25mm
Plate width	83.97 ± 0.25mm
Plate height	23.20 ± 0.25mm
Well depth	20.20 ± 0.10mm
Well diameter	5.46 ± 0.10mm
Total well capacity	0.3ml (300µl)
Working volume	<0.2ml (200µl)
Distance to center of A1 from top edge	10.495 ± 0.25mm
Distance to center of A1 from left edge	12.63 ± 0.25mm
Pitch (distance between A1 and A2)	9.00mm

Compatibility

ABI®/LIFE TECHNOLOGIES/THERMO FISHER SCIENTIFIC
- Thermal Cyclers (96 well standard block): Verity | Proflex | Simpliamp | GeneAmp^® 2700/2720/9600/9700
- qPCR Cyclers (96 well standard block): 7000 | 7300 | 7500 | 7700 | 7900 HT | QuantStudio™ 3/5/6/7/12K | ViiA7™
- Sequencers (96 well block): 3100 | 3130XL | 3500 | 3500XL | 3730 | 3730XL
AGILENT/STRATAGENE
- qPCR Cyclers (96 well block): Mx4000™
SENSOQUEST
- Thermal Cyclers (96 well block): Labcycler

For recommended plate options depending on manufacturer, block type, and instrument please refer to the Azenta Life Sciences Plate Instrument Compatibility Table.

Ordering Information

Use these part numbers to request a quote, a sample or to contact an expert:

Part number	Description
FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI^® Style Standard profile, 0.2ml polypropylene wells, polycarbonate frame with upstand, cut corner A12, working volume: <200µl, total well capacity: 300µl; designed for use on ABI^® instruments
4ti-0730	clear wells, purple frame; 50 plates
4ti-0730/B	clear wells, blue frame; 50 plates
4ti-0730/C	clear wells, clear frame; 50 plates
4ti-0730/C/10P	clear wells, clear frame; 10 plates
4ti-0730/G	clear wells, green frame; 50 plates
4ti-0730/R	clear wells, red frame; 50 plates
4ti-0730/X	clear wells, black frame; 50 plates
4ti-OX730C/SBC	4ti-0730/C for forensic use, ethylene oxide treated, single barcoded; 20 plates
Combi packs
4ti-0730/C/0565	4ti-0730/C + Optically Clear Windowed qPCR Seal (4ti-0565); 50 plates + 50 seals

Resources

Consumable Effects on Low Volume TaqMan® Assays for Quantitative PCR on Roche LightCycler® 480

Application Notes & Tech Notes

Miniaturization of a qPCR Assay with Reduced Reagent Volumes to Achieve Cost Savings in Gene Expression Analyses in Rumen Epithelium

Application Notes & Tech Notes

PCR Plate-Instrument Compatibility

Brochures & Sell Sheets

Azenta Life Sciences Sample Consumables and Instruments Catalog

Catalogs