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  • 4ti-0770 | FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style | Front
  • 4ti-0770 | FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style
  • 4ti-0770/B | FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style | Front
  • 4ti-0770/B | FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style
  • 4ti-0770/C | FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style | Front
  • 4ti-0772/C | FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style
  • 4ti-0770/G | FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style | Front
  • 4ti-0772/G | FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style
  • 4ti-0770/R | FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style | Front
  • 4ti-0772/R | FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style
  • 4ti-0770/X | FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style | Front
  • 4ti-0772/X | FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style
  • 4ti-0771 | FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style | Front
  • 4ti-0771 | FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style
  • 4ti-0772 | FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style | Front
  • 4ti-0772 | FrameStar® 96 Well Semi-Skirted PCR Plate, ABI® Style

FrameStar® 96 Well Semi-Skirted PCR Plate, ABI® Style

Standard profile, 0.2ml polypropylene wells, polycarbonate frame, cut corner A12; working volume: <200µl, total well capacity: 300µl; designed for use on all major cyclers, including ABI® instruments with standard 96 well blocks

  • Available barcoded on request
  • Ultra-low DNA binding option available

Place a Request

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BUY ONLINE
Online availability to US only

Jump to:
  • Overview
  • Low DNA Binding Products
  • Key Features
  • Specifications
  • Compatibility
  • Ordering Information
  • Resources
  • Related Products
  • Place a Request

Overview

Specifically designed to be directly compatible with all major thermal cyclers, this plate can be used directly in ABI® 96 well instruments without the need for any adapters. The rigid FrameStar 2-component design eliminates warping and distortion during PCR, making it ideal for use with robotic systems. The semi-skirt allows for labeling or barcoding for sample tracking. Learn more about the features and benefits of the FrameStar range.

Low DNA Binding Products

  • Azenta Life Sciences offer low binding products, recommended to support sensitive techniques such as qPCR and Next Generation Sequencing (NGS), as well as storage of samples.
  • Manufactured using selected low-bind polymers, DNA loss is minimized during incubation and sample transfer, making it the ideal solution for use in applications with low DNA input.
  • Low binding feature results from selected low-bind polymers, no coating is used to achieve the binding characteristics. No contamination or modification of the samples.
  • Tested under a broad temperature range. Maximum DNA recovery after low temperature storage and high temperature incubations.
  • No detectable DNA loss during incubation steps and sample transfer in NGS sample prep and library construction. Ideally suited for sensitive applications with ultra-low nucleic acid input.

Key Features

  • Quality
    • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
  • Wells
    • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
    • <0.2ml (200µl) working volume, 0.3ml (300µl) total well capacity
  • Frame
    • Cut corner at A12
    • Rigid polycarbonate frame for added mechanical stability
    • Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
    • Improved seal integrity due to thermal stability of frame
    • Alphanumeric grid reference to aid well and sample identification
  • Use
    • Perfect for ABI® thermal cyclers & sequencers
    • Recommended for low volume PCR
    • Ideal for use with robotic systems
    • Compatible with standard multichannel pipettes
  • Options
    • Available with the following frame color options for the clear well variety: purple, blue, clear, green, red, and black
    • Also available as a black frame with white wells for optimum signal-to-noise ratio when using fluorescent based assays
    • Also available as a clear frame with frosted wells for use with ABI®/LifeTechnologies® qPCR instruments
    • Ultra-low DNA binding option available (4ti-LB0770/C) for sensitive applications with ultra-low DNA input and for maximum DNA recovery after low temperature storage and high temperature incubation; learn more about our low binding range
    • Ethylene oxide treated option available (4ti-OX770C/SBC) for forensic use; learn more about our ethylene oxide treated range
    • Available barcoded upon request; learn more about our barcoding options
    • FrameStar 96 Lid (4ti-0289) available

Specifications

Parameter FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style
Plate length 124.26 ± 0.25mm
Plate width 83.97 ± 0.25mm
Plate height 20.70 ± 0.25mm
Well depth 20.20 ± 0.10mm
Well diameter 5.46 ± 0.10mm
Total well capacity 0.3ml (300µl)
Working volume 0.2ml (200µl)
Distance to center of A1 from top edge 10.495 ± 0.25mm
Distance to center of A1 from left edge 12.63 ± 0.25mm
Pitch (distance between A1 and A2) 9.00mm

Compatibility

  • ABI®/LIFE TECHNOLOGIES/THERMO FISHER SCIENTIFIC
    • Thermal Cyclers (96 well standard block): Verity | Proflex | Simpliamp | GeneAmp® 2700/2720/9600/9700
    • qPCR Cyclers (96 well standard block): 7000 | 7300 | 7500 | 7700 | 7900 HT | QuantStudio™ 3/5/6/7/12K | ViiA7™
    • Sequencers (96 well block): 3100 | 3130XL | 3500 | 3500XL | 3730 | 3730XL
  • AGILENT/STRATAGENE
    • qPCR Cyclers (96 well block): Mx3000P™ | Mx3005P™ | Mx4000™
    • Fragment Analyzer Systems: 5200 | 5300 | 5400
  • ANALYTIK JENA/BIOMETRA
    • Thermal Cyclers (96 well block): Flexcycler2 | T1 Thermocycler | Tgradient | Tone | Tadvanced | TProfessional (Standard/Basic) Gradient / XL | Trobot 96 | SpeedCycler2 (SP, SPR)
    • qPCR Cyclers (96 well block): qTOWER3/G/touch | Toptical
  • BIOER TECHNOLOGIES
    • Thermal Cyclers (96 well block): Gene Touch 96
  • BIO-RAD
    • Thermal Cyclers (96 well block): C1000 Touch | S1000 | iCycler™ | MyCycler™ | T100
    • qPCR Cyclers (96 well block): MyiQ™ | iCycler™ IQ/IQ 4/IQ 5
  • BIO-RAD MJ RESEARCH
    • Thermal Cyclers (96 well block): PCT100™/200™/220™/221™/225™/240™
    • qPCR Cyclers (96 well block): Chromo4™
  • CORBETT RESEARCH
    • Thermal Cyclers (96 well block): (Qiagen) Palm Cycler
  • EPPENDORF
    • Thermal Cyclers (96 well block): MasterCycler® ep/ep gradient/Pro/Pro S/nexus/nexus gradient/nexus SX1/nexus GSX1
    • qPCR Cyclers (96 well block): Mastercycler™ ep realplex
  • PEQLAB/VWR
    • Thermal Cyclers (96 well block): peqSTAR 96X
  • SENSOQUEST
    • Thermal Cyclers (96 well block): Labcycler
  • TAKARA
    • Thermal Cyclers (96 well block): Dice touch | Gradient
  • TECHNE
    • Thermal Cyclers (96 well block): Prime | PrimeG | Prime Elite | Prime Elite Satellite | PCRmax Alpha cycler 1/2/4 | TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene

For recommended plate options depending on manufacturer, block type, and instrument please refer to the Azenta Life Sciences Plate Instrument Compatibility Table.

Ordering Information

Use these part numbers to request a quote, a sample or to contact an expert:

Part number Description
FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style
Standard profile, 0.2ml polypropylene wells, polycarbonate frame, cut corner A12; working volume: <200µl, total well capacity: 300µl; designed for use on all major cyclers, including ABI® instruments with standard 96 well blocks
4ti-0770 clear wells, purple frame; 50 plates
4ti-0770/B clear wells, blue frame; 50 plates
4ti-0770/C
BUY ONLINE*
clear wells, clear frame; 50 plates
4ti-0770/C/10P clear wells, clear frame; 10 plates
4ti-0770/G clear wells, green frame; 50 plates
4ti-0770/R clear wells, red frame; 50 plates
4ti-0770/X clear wells, black frame; 50 plates
4ti-0771 white wells, black frame; 50 plates
4ti-0772 frosted wells, clear frame; 50 plates
4ti-0772/10P frosted wells, clear frame; 10 plates
4ti-OX770C/SBC 4ti-0770/C for forensic use, ethylene oxide treated, single barcoded; 20 plates
4ti-LB0770/C 4ti-0770/C, with low DNA binding properties; 50 plates
Lids
4ti-0289 FrameStar 96 Lid (4ti-0289), for use with the FrameStar 96 Well Semi-Skirted Plates, ABI® Style range; 50 lids

*Online availability to US only

Resources

Consumable Effects on Low Volume TaqMan® Assays for Quantitative PCR on Roche LightCycler® 480

Consumable Effects on Low Volume TaqMan® Assays for Quantitative PCR on Roche LightCycler® 480

Application Notes & Tech Notes
Miniaturization of a qPCR Assay with Reduced Reagent Volumes to Achieve Cost Savings in Gene Expression Analyses in Rumen Epithelium

Miniaturization of a qPCR Assay with Reduced Reagent Volumes to Achieve Cost Savings in Gene Expression Analyses in Rumen Epithelium

Application Notes & Tech Notes
FrameStar® qPCR Plates with Frosted Wells

FrameStar® qPCR Plates with Frosted Wells

Brochures & Sell Sheets
Low DNA Binding Products Brochure

Low DNA Binding Products Brochure

Brochures & Sell Sheets
PCR Plate-Instrument Compatibility

PCR Plate-Instrument Compatibility

Brochures & Sell Sheets
Azenta Life Sciences Sample Consumables and Instruments Catalog

Azenta Life Sciences Sample Consumables and Instruments Catalog

Catalogs
Avoiding DNA Adsorption to Tube Walls in NGS Sample Preparation

Avoiding DNA Adsorption to Tube Walls in NGS Sample Preparation

Publications & Posters
FrameStar® 96 Well Semi-Skirted PCR Plate, ABI® Style Technical Drawing

FrameStar® 96 Well Semi-Skirted PCR Plate, ABI® Style Technical Drawing

Technical Drawings
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Related Products

Similar Products

See below for a selection of products in the same range:

4ti-0910/C | FrameStar 96 Well Semi-Skirted PCR Plate, ABI® FastPlate Style | Front

FrameStar® 96 Well Semi-Skirted PCR Plate, ABI® FastPlate Style

On

Low profile, 0.1ml polypropylene wells, polycarbonate frame with upstand, cut corner A1, working volume: <100µl, total well capacity: 200µl; designed for use on ABI® Fast Block cycler

4ti-0730/G | FrameStar 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style | Front

FrameStar® 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style

On

Standard profile, 0.2ml polypropylene wells, polycarbonate frame with upstand, cut corner A12, working volume: <200µl, total well capacity: 300µl; designed for use on ABI® instruments

4ti-0735 | 96 Well Semi-Skirted PCR Plate With Upstand, ABI Style | Front

96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style

Off

Standard profile, 0.2ml wells, polypropylene, cut corner A12, working volume: <200µl, total well capacity: 300µl; designed for use on ABI® thermal cyclers

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Compatible Products

See below for a selection of compatible products:

4ti-0665 | Automated Roll Heat Sealer (formerly a4S)

Automated Roll Heat Sealer (formerly a4S)

BUY ONLINE
On

The perfect solution for automated heat sealing of microplates, PCR plates, assay plates and storage plates used in medium to high-throughput or full automation applications

59-2000 | Semi-Automated Sheet Heat Sealer (formerly IntelliXseal SA) | Side | Holder & Plate Detail

Semi-Automated Sheet Heat Sealer (formerly IntelliXseal™ SA)

BUY ONLINE
On

Semi-automated sheet heat sealer compatible with a wide range of seals and microplates, PCR and assay plates, as well as storage plates of differing designs and heights

4ti-0565 | Optically Clear Windowed qPCR Seal | Peeling Detail

Optically Clear Windowed qPCR Seal

Off

Adhesive film with 96 optically clear windows, peelable; suitable for qPCR and optical applications

4ti-0500 | PCR Seal

PCR Seal

BUY ONLINE
On

Clear adhesive film, strong adhesive, peelable; suitable for PCR and optical applications; available as a full sheet, perforated for tearing into 8 well strips or 12 well strips

PCR Plate Lids | Group

PCR Plate Lid

Off

Rigid polystyrene lids for PCR, designed to give a quick and easy sealing solution to protect samples from contamination and evaporation

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Place a Request

Request a Quote, Free Samples, or an Expert Consultation

Want to learn more about this product? Speak to one of our experts, or request a quote or free samples by filling out and submitting the form.

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