FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity

Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H12, working volume: <100µl, total well capacity: 200µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block); extra white wells for improved sensitivity in fluorescent assays

  • Available barcoded on request
Overview

Our FrameStar Roche Style plates are designed to achieve optimized assay conditions on the Roche LightCycler® 96 and 480 (with 96 well block). This particular style of plate is in a low profile 96-well format; perfect for reaction volumes of 10-100µl.

The wells are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well. This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction.

This is especially recommended for reaction volumes below 20µl. The extra white wells allow for increased sensitivity in fluorescent assays. The semi-skirted frame allows for labeling or barcoding.

Key Features
  • Quality
    • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
  • Wells
    • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
    • <0.1ml (100µl) working volume, 0.2ml (200µl) total well capacity
  • Frame
    • Rigid polycarbonate frame for added mechanical stability
    • Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
    • Improved seal integrity due to thermal stability of frame
    • Alphanumeric grid reference to aid well and sample identification
  • Use
    • Compatible with the Roche LightCycler® 96 and 480 (with 96 well block)
    • Recommended for low volume PCR
    • Compatible with standard multichannel pipettes
    • Ideal for use with robotic systems
  • Options
    • Plates are available with a clear polycarbonate frame and extra white wells for improved sensitivity when using fluorescent based assays
    • Combi packs available with qPCR Seal (4ti-0560)
    • Available barcoded upon request; learn more about our barcoding options
Specifications
Parameter FrameStar 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity
Plate length 127.70 ± 0.25mm
Plate width 85.48 ± 0.25mm
Plate height 15.60 ± 0.25mm
Well depth 15.10 ± 0.10mm
Well diameter 5.50 ± 0.10mm
Total well capacity 0.2ml (200µl)
Working volume <0.1ml (100µl)
Distance to center of A1 from top edge 11.24 ± 0.25mm
Distance to center of A1 from left edge 14.38 ± 0.25mm
Pitch (distance between A1 and A2) 9.00mm

Ct values comparison

  • Ct values of data run on a Roche LightCycler® 480, comparing 4titude FrameStar Roche Style, High Sensitivity plates (4ti-0954) and Competitor R equivalent plates
  • The new 4titude 4ti-0954 plate out performs the competitor plate with significantly earlier Ct value
FrameStar Roche Style, High Sensitivity Plate
Ct value 17.23
Δ Ct -3.05
Competitor R equivalent plates
Ct value 20.28
Δ Ct 0
Compatibility
  • ROCHE
    • qPCR Cyclers (96 well block): LC96 | LC480

For recommended plate options depending on manufacturer, block type, and instrument please refer to the Azenta Life Sciences Plate Instrument Compatibility Table.

Ordering Information

Use these part numbers to request a quote, a sample or to contact an expert:

Part number Description
FrameStar 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity Plate
Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H12, working volume: <100µl, total well capacity: 200µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block); extra white wells for improved sensitivity in fluorescent assays
4ti-0954 extra white wells, clear frame; 50 plates
Combi packs
4ti-0954/0560 4ti-0954 + qPCR Seal (4ti-0560); 50 plates + 50 seals