FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity
Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H12, working volume: <100µl, total well capacity: 200µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block); extra white wells for improved sensitivity in fluorescent assays
- Available barcoded on request
Our FrameStar Roche Style plates are designed to achieve optimized assay conditions on the Roche LightCycler® 96 and 480 (with 96 well block). This particular style of plate is in a low profile 96-well format; perfect for reaction volumes of 10-100µl.
The wells are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well. This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction.
This is especially recommended for reaction volumes below 20µl. The extra white wells allow for increased sensitivity in fluorescent assays. The semi-skirted frame allows for labeling or barcoding.
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Quality
- Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
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Wells
- Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
- <0.1ml (100µl) working volume, 0.2ml (200µl) total well capacity
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Frame
- Rigid polycarbonate frame for added mechanical stability
- Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
- Improved seal integrity due to thermal stability of frame
- Alphanumeric grid reference to aid well and sample identification
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Use
- Compatible with the Roche LightCycler® 96 and 480 (with 96 well block)
- Recommended for low volume PCR
- Compatible with standard multichannel pipettes
- Ideal for use with robotic systems
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Options
- Plates are available with a clear polycarbonate frame and extra white wells for improved sensitivity when using fluorescent based assays
- Combi packs available with qPCR Seal (4ti-0560)
- Available barcoded upon request; learn more about our barcoding options
| Parameter | FrameStar 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity |
| Plate length | 127.70 ± 0.25mm |
| Plate width | 85.48 ± 0.25mm |
| Plate height | 15.60 ± 0.25mm |
| Well depth | 15.10 ± 0.10mm |
| Well diameter | 5.50 ± 0.10mm |
| Total well capacity | 0.2ml (200µl) |
| Working volume | <0.1ml (100µl) |
| Distance to center of A1 from top edge | 11.24 ± 0.25mm |
| Distance to center of A1 from left edge | 14.38 ± 0.25mm |
| Pitch (distance between A1 and A2) | 9.00mm |
Ct values comparison
- Ct values of data run on a Roche LightCycler® 480, comparing 4titude FrameStar Roche Style, High Sensitivity plates (4ti-0954) and Competitor R equivalent plates
- The new 4titude 4ti-0954 plate out performs the competitor plate with significantly earlier Ct value
| FrameStar Roche Style, High Sensitivity Plate | |
| Ct value | 17.23 |
| Δ Ct | -3.05 |
| Competitor R equivalent plates | |
| Ct value | 20.28 |
| Δ Ct | 0 |
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ROCHE
- qPCR Cyclers (96 well block): LC96 | LC480
For recommended plate options depending on manufacturer, block type, and instrument please refer to the Azenta Life Sciences Plate Instrument Compatibility Table.
Use these part numbers to request a quote, a sample or to contact an expert:
| Part number | Description |
| FrameStar 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity Plate Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H12, working volume: <100µl, total well capacity: 200µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block); extra white wells for improved sensitivity in fluorescent assays |
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| 4ti-0954 | extra white wells, clear frame; 50 plates |
| Combi packs | |
| 4ti-0954/0560 | 4ti-0954 + qPCR Seal (4ti-0560); 50 plates + 50 seals |
See below for a selection of products in the same range:
See below for a selection of compatible sealing options:
