FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style

Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H12; working volume: <100µl, total well capacity: 200µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block)

  • Available barcoded on request
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Overview

Our FrameStar Roche Style plates are designed to achieve optimized assay conditions on the Roche LightCycler® 96 and 480 (with 96 well block). This particular style of plate is in a low profile 96-well format; perfect for reaction volumes of 10-100µl.

The wells are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well. This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction.

This is especially recommended for reaction volumes below 20µl. The semi-skirted frame allows for labeling or barcoding.

Key Features
  • Quality
    • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
  • Wells
    • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
    • <0.1ml (100µl) working volume, 0.2ml (200µl) total well capacity
  • Frame
    • Rigid polycarbonate frame for added mechanical stability
    • Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
    • Improved seal integrity due to thermal stability of frame
    • Alphanumeric grid reference to aid well and sample identification
  • Use
    • Compatible with the Roche LightCycler® 96 and 480 (with 96 well block)
    • Recommended for low volume PCR
    • Ideal for use with robotic systems
    • Compatible with standard multichannel pipettes
  • Options
    • Standard plate comes with clear polypropylene wells on a clear polycarbonate frame
    • Also available with white wells for optimum signal-to-noise ratio when using fluorescent based assays
    • Available barcoded upon request; learn more about our barcoding options
    • Combi packs available with qPCR Seal (4ti-0560) and (for 4ti-0951) with Optically Clear Windowed qPCR Seal (4ti-0564)
Specifications
Parameter FrameStar 96 Well Semi-Skirted PCR Plate, Roche Style
Plate length 127.70 ± 0.25mm
Plate width 85.48 ± 0.25mm
Plate height 15.60 ± 0.25mm
Well depth 15.10 ± 0.10mm
Well diameter 5.50 ± 0.10mm
Total well capacity 0.2ml (200µl)
Working volume <0.1ml (100µl)
Distance to center of A1 from top edge 11.24 ± 0.25mm
Distance to center of A1 from left edge 14.38 ± 0.25mm
Pitch (distance between A1 and A2) 9.00mm
Compatibility
  • ROCHE
    • qPCR Cyclers (96 well block): LC96 | LC480

For recommended plate options depending on manufacturer, block type, and instrument please refer to the Azenta Life Sciences Plate Instrument Compatibility Table.

Ordering Information

Use these part numbers to request a quote, a sample or to contact an expert:

Part number Description
FrameStar 96 Well Semi-Skirted PCR Plate, Roche Style
Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H12; working volume: <100µl, total well capacity: 200µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block)
4ti-0950/C clear wells, clear frame; 50 plates
4ti-0951
BUY NOW (US ONLY)
white wells, clear frame; 50 plates
Combi packs
4ti-0952 4ti-0951 + qPCR Seal (4ti-0560); 50 plates + 50 seals
4ti-0953 4ti-0950/C + qPCR Seal (4ti-0560); 50 plates + 50 seals
4ti-0951/0565 4ti-0951 + Optically Clear Windowed qPCR Seal (4ti-0565); 50 plates + 50 seals