FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style
Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H12; working volume: <100µl, total well capacity: 200µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block)
- Available barcoded on request
Our FrameStar Roche Style plates are designed to achieve optimized assay conditions on the Roche LightCycler® 96 and 480 (with 96 well block). This particular style of plate is in a low profile 96-well format; perfect for reaction volumes of 10-100µl.
The wells are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well. This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction.
This is especially recommended for reaction volumes below 20µl. The semi-skirted frame allows for labeling or barcoding.
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Quality
- Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
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Wells
- Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
- <0.1ml (100µl) working volume, 0.2ml (200µl) total well capacity
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Frame
- Rigid polycarbonate frame for added mechanical stability
- Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
- Improved seal integrity due to thermal stability of frame
- Alphanumeric grid reference to aid well and sample identification
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Use
- Compatible with the Roche LightCycler® 96 and 480 (with 96 well block)
- Recommended for low volume PCR
- Ideal for use with robotic systems
- Compatible with standard multichannel pipettes
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Options
- Standard plate comes with clear polypropylene wells on a clear polycarbonate frame
- Also available with white wells for optimum signal-to-noise ratio when using fluorescent based assays
- Available barcoded upon request; learn more about our barcoding options
- Combi packs available with qPCR Seal (4ti-0560) and (for 4ti-0951) with Optically Clear Windowed qPCR Seal (4ti-0564)
| Parameter | FrameStar 96 Well Semi-Skirted PCR Plate, Roche Style |
| Plate length | 127.70 ± 0.25mm |
| Plate width | 85.48 ± 0.25mm |
| Plate height | 15.60 ± 0.25mm |
| Well depth | 15.10 ± 0.10mm |
| Well diameter | 5.50 ± 0.10mm |
| Total well capacity | 0.2ml (200µl) |
| Working volume | <0.1ml (100µl) |
| Distance to center of A1 from top edge | 11.24 ± 0.25mm |
| Distance to center of A1 from left edge | 14.38 ± 0.25mm |
| Pitch (distance between A1 and A2) | 9.00mm |
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ROCHE
- qPCR Cyclers (96 well block): LC96 | LC480
For recommended plate options depending on manufacturer, block type, and instrument please refer to the Azenta Life Sciences Plate Instrument Compatibility Table.
Use these part numbers to request a quote, a sample or to contact an expert:
| Part number | Description |
| FrameStar 96 Well Semi-Skirted PCR Plate, Roche Style Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H12; working volume: <100µl, total well capacity: 200µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block) |
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| 4ti-0950/C | clear wells, clear frame; 50 plates |
| 4ti-0951 BUY NOW (US ONLY) |
white wells, clear frame; 50 plates |
| Combi packs | |
| 4ti-0952 | 4ti-0951 + qPCR Seal (4ti-0560); 50 plates + 50 seals |
| 4ti-0953 | 4ti-0950/C + qPCR Seal (4ti-0560); 50 plates + 50 seals |
| 4ti-0951/0565 | 4ti-0951 + Optically Clear Windowed qPCR Seal (4ti-0565); 50 plates + 50 seals |
See below for a selection of products in the same range:
See below for a selection of compatible sealing options:
