96 Well Semi-Skirted PCR Plate, Roche Style

Low profile, 0.1ml wells, polypropylene, cut corner H12, working volume: <100µl, total well capacity: 200µl; designed for use on Roche LightCycler 480®

  • Available barcoded on request
Overview

Low profile, 0.1 ml wells, polypropylene, cut corner H12, working volume: <100µl, total well capacity: 200µl; designed for use on Roche LightCycler 480. This Roche Style plate is designed to achieve optimized assay conditions on the Roche LightCycler 480. This particular style of plate is in a low profile 96-well format, perfect for reaction volumes of 10-100µl.

The wells are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well. This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction. This is especially recommended for reaction volumes below 20µl.

Key Features
  • Quality
    • Subject to a strict QC procedure which includes a visual check and leak testing of every tube
    • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
  • Wells
    • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
    • <0.1ml (100µl) working volume, 0.2ml (200µl) total well capacity
    • Maximum thermal conductivity for efficient heat transfer and precise thermal cycling
  • Frame
    • Alphanumeric grid reference to aid well and sample identification
  • Use
    • Optimized for use with the Roche LightCycler 480
    • Recommended for low volume PCR
    • Compatible with standard multichannel pipettes
  • Options
    • Available with white wells give optimum signal-to-noise ratio when using fluorescent-based assays
    • Combi packs available with qPCR Seal (0560)
    • Available barcoded upon request; learn more about our barcoding options
    • Available as a FrameStar® 2-component PCR plate with the same dimensions, but with the added benefits of a polycarbonate frame that eliminates plate distortion and warping during the PCR process, leading to less evaporation and sample loss
Specifications
Parameter 96 Well Semi-Skirted PCR Plate, Roche Style
Plate length 127.70 ± 0.25mm
Plate width 85.48 ± 0.25mm
Plate height 15.60 ± 0.10mm
Well depth 15.10 ± 0.10mm
Well diameter 5.50 ± 0.10mm
Total well capacity 0.2ml (200µl)
Working volume <0.1ml (100µl)
Distance to center of A1 from top edge 11.24 ± 0.25mm
Distance to center of A1 from left edge 14.38 ± 0.25mm
Pitch (distance between A1 and A2) 9.00mm
Compatibility
  • ROCHE
    • qPCR Cyclers (96 well block): LC96 | LC480

For recommended plate options depending on manufacturer, block type, and instrument please refer to the Azenta Life Sciences Plate Instrument Compatibility Table.

Ordering Information

Use these part numbers to request a quote, a sample or to contact an expert:

Part number Description
96 Well Semi-Skirted PCR Plate, Roche Style
Low profile, 0.1ml wells, polypropylene, cut corner H12, working volume: <100µl, total well capacity: 200µl; designed for use on Roche LightCycler 480
4ti-0955 white; 50 plates
Combi packs
4ti-0955/0560 4ti-0955 + qPCR Seal (4ti-0560); 50 plates + 50 seals