Individual Access 96 Well Skirted Flat Bottom PCR Plate, 2D Coded

96 well skirted plates, unique on the market, with individually removable and sealable flat bottom 2D coded tubes, designed for (q)PCR, microscopy and small volume sample storage. Low profile wells, recommended for reaction volumes <20µl. Ideal for use with robotic systems.

PP wells, rigid white PC frame; cut corner H1
<100µl working volume, 180µl total well capacity
Same tube fit for microscopy, (q)PCR, storage
2D coded bottom for reliable sample identification

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Product Details

96 well, fully skirted, low profile format with each tube inserted separately into the plate frame, allowing for selection and removal of individual tubes from the plate. Each tube clicks into place in the frame ensuring tubes are secure once inserted.

We have now extended our Individual Access range to include PCR plates with 2D coded flat bottoms for applications that require superior sample tracking. In applications where it is essential for samples to be tracked throughout their processing, e.g. for diagnostic assays on clinical samples, tube labeling is a far safer and reliable method compared to cap or seal labeling (which can be misplaced, damaged or rendered unreadable after piercing).

Our plates have a unique 2D code printed on the bottom of the well and a linear barcode on the side of the plate to allow for identification of both the plate and individual tubes within. The rigid polycarbonate skirt of this design eliminates warping and distortion during PCR. Together with the SBS footprint, this makes the plate suitable for use with robotic systems, ideal for both small and high throughput labs.

Empty frames are available for tubes to be transferred to. The frames can be re-used multiple times, depending on the application. Sample tubes can be identified by the unique 2D code on the base of the wells, removed from the Individual Access plate quickly and easily, and moved to another Individual Access frame for downstream processing or storage.

Quick selection of desired tubes reduces the time needed for the sample plate to be out of the freezer and therefore reduces the chance of other wells defrosting in the meantime. Flat bottom PCR plates can be stacked, allowing for optimal use of freezer space as multiple plates can be housed in the same space as a storage rack. The 2D codes on the bottom of the wells can be read by most 2D datamatrix readers including Azenta Life Sciences Whole Rack Scanners The codes are highly scratch resistant and can withstand cold storage (-80°C), temperatures up to 100°C and solvents such as DMSO. 2D datamatrix codes utilize data redundancy so even if codes are partly destroyed, the information will still be retained. Each code is tested for readability and guaranteed to be unique.

Key Features

Quality

Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen

Tubes

Individually removable from the rigid frame, while the rest of the tubes can remain in storage, reducing freeze-thawing of the whole plate
Thin walled tubes are made from PP for optimal results in (q)PCR whilst also being suitable for storage and incubation
<100µl working volume, <180µl total well capacity

Frame

Rigid polycarbonate frame provides mechanical stability of the plate
These plates are labeled with linear Code 128-format barcodes to aid identification and traceability for your samples
Alphanumeric grid reference to aid well and sample identification

Use

Suitable for small volume sample storage, allowing for optimal use of freezer space as multiple plates can be housed in the same space as a storage rack
Ideal for use with robotic systems
Fewer transfer steps as the same tube can be used for microscopy, (q)PCR and storage
Each tube can be individually heat sealed using the Individual Access Pierce Heat Seal Strong, such as the contents of each tube is left secure from cross contamination and evaporation for storage and transfer
Each tube can still be separated after sealing

Options

Also available as a Individual Access optical bottom plate (4ti-0970/RA) with black frame, ideal for microscopy and PCR
Empty frames are available for tubes to be transferred to; frames can be re-used multiple times, depending on the application
We also offer customization of the Individual Access Plates such as color coding of the individual wells; please contact an expert for details
The plate can be sealed in one step using Individual Access Pierce Heat Seal Strong (4ti-05381/RA) on the Semi-Automated Sheet Heat Sealer (using the 59-2005 Individual Access adapter), resulting in individually sealed tubes with pierceable seals to allow for sample access

Specifications

	Individual Access 96 Well Skirted Flat Bottom PCR Plate, 2D Coded
Plate length	127.76 ± 0.25mm
Plate width	85.48 ± 0.25mm
Plate height	15.50 ± 0.25mm
Well depth	12 ± 0.10mm
Well diameter	5.46 ± 0.10mm
Total well capacity	180µl
Working volume	<0.1ml (100µl)
Distance to center of A1 from top edge	11.24 ± 0.25mm
Distance to center of A1 from left edge	14.38 ± 0.25mm
Pitch (distance between A1 and A2)	9.00mm
Code size	12 x 12 px; 2 x 2mm
Code format	white on black
Code content	8 digit numeric

Product Documentation

Individual Access 96 Well Skirted Flat Bottom PCR Plate, 2D Coded Technical Drawing

Differences in tube format between the standard conical PCR tube and the flat bottom tubes — Figure 1

Figure 1 (click on image to expand) illustrates the differences in tube format between the standard conical PCR tube and the flat bottom tubes
The surface area of the optical base is 7.74mm², with a thickness of 0.5mm
The total volume of a conical well is 0.2ml compared to 0.18ml for flat bottom tubes

Compatibility

AGILENT/STRATAGENE

Thermal Cyclers (96 well block): Robocycler Gradient
qPCR Cyclers (96 well block): AriaMx

BIO-RAD

Thermal Cyclers (96 well block): C1000 Touch | S1000
qPCR Cyclers (96 well block): CFX96 Touch | CFX96 Touch Deep Well | CFX connect

BIO-RAD MJ RESEARCH

Thermal Cyclers (96 well block): PCT100™/200™/220™/221™/225™/240™
qPCR Cyclers (96 well block): Chromo4™ | Opticon2™

EPPENDORF

Thermal Cyclers (96 well block): MasterCycler^® ep/ep gradient/Pro/Pro S/nexus/nexus gradient/nexus SX1/nexus GSX1
qPCR Cyclers (96 well block): MasterCycler™ ep realplex

PEQLAB/VWR

Thermal Cyclers (96 well block): peqSTAR 96X

SENSOQUEST

Thermal Cyclers (96 well block): Labcycler

TECHNE

Thermal Cyclers (96 well block): TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene
qPCR Cyclers (96 well block): Quantica

For recommended plate options depending on manufacturer, block type, and instrument please refer to the Azenta Life Sciences Plate Instrument Compatibility Table.

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