Our optically clear tissue culture plates are assembled using unique patented laser welding technology which reduces autofluorescence and does not inhibit cell growth (Figure 1 and Figure 2, click on image to expand). Other competitors assemble clear base microplates by gluing a clear film to the frame or heat-welding the components together. Both techniques can cause problems with cell growth and the subsequent microscopic or fluorometric analysis.
Heat welding of the two plate components under high pressure results in autofluorescence at the well edges called “halo effect”. Gluing uses organic solvents within the adhesives, which can also cause autofluorescence. These solvents can also have cytotoxic effects, which may lead to inhibition of cell growth or even cell death. Additionally, incomplete glue lines or weld lines often result in well-to-well leakage.