Paper 6 of the Cryopreservation series
Exposure of cryopreserved biological material to ambient temperatures may reduce the functionality and viability of the stored material, particularly when sample temperature rises above the glass transition temperature (Tg) of the material.
Most commonly, samples experience exposure to ambient temperatures during processing, shipment, and transfer between storage containers.
It is important to understand how different types of samples warm and at what rate they warm to inform best practices for sample handling and storage.
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