Paper 1 of the Cryopreservation series
Many biological samples contained inside cryoboxes are stored in LN2 vapor-phase freezers at temperatures below -150°C to preserve their viability. The underlying assumption is that biological samples show highly reduced degradation and metabolic activity while below Tg (the glass transition temperature).
However, every time a cryobox is manipulated or temporarily removed from a LN2 freezer the enclosed samples experience thermal excursions. They could be harmed by inadvertently crossing the Tg threshold or by exposure to excessive thermal excursions.
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