Thermal Protection: Protecting Excursions of Cryogenically Frozen Vials During Transient Warming & Best Practices to Stay Below Tg,H2O

Paper 1 of the Cryopreservation series

In this White Paper

Best Practices for Keeping Cryogenic Samples Below Glass Transition

Many biological samples contained inside cryoboxes are stored in LN2 vapor-phase freezers at temperatures below -150°C to preserve their viability. The underlying assumption is that biological samples show highly reduced degradation and metabolic activity while below Tg (the glass transition temperature).

However, every time a cryobox is manipulated or temporarily removed from a LN2 freezer the enclosed samples experience thermal excursions. They could be harmed by inadvertently crossing the Tg threshold or by exposure to excessive thermal excursions.

Learn about the impact of temperature variations on cryogenically frozen samples and how to protect these samples from harm!