Preserving the Innocents: Biosample Storage at -190ºC
Paper 4 of the Cryopreservation series
It seems to be universally accepted that storing at -190°C in a liquid nitrogen (LN2) vapor-phase freezer is the best technique for biosample preservation.
Azenta Life Sciences decided to explore this idea further, looking specifically at what happens to the innocent samples that are pulled from the freezer, the ones neither used nor touched, but going along for the transient warming ride.
Samples may be removed from the freezer countless times during storage. The goal of this paper was to understand temperature excursion and the related warming rates more closely.
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