Thermal Protection: Protecting Excursions of Cryogenically Frozen Vials During Transient Warming & Best Practices to Stay Below Tg,H2o

Paper 1 of the Cryopreservation series

Many biological samples contained inside cryoboxes are stored in LN2 vapor-phase freezers at temperatures below -150°C to preserve their viability. The underlying assumption is that biological samples show highly reduced degradation and metabolic activity while below Tg (the glass transition temperature).

However, every time a cryobox is manipulated or temporarily removed from a LN2 freezer the enclosed samples experience thermal excursions. They could be harmed by inadvertently crossing the Tg threshold or by exposure to excessive thermal excursions.

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