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CoolCell® Cell Freezing Containers

Alcohol-Free Cell Freezing Containers: Standardized, Passive Controlled Rate Freezing

CoolCell Alcohol-Free Cell Freezing Containers ensure standardized controlled-rate -1˚C/minute cell freezing in a -80˚C freezer - without alcohol or any fluids. Proven for use with a variety of cell types including stem cells, primary cells, PBMC cell lines, insect cells, yeast and others.

The patent-pending CoolCell Alcohol-Free Cell Freezing Container technology utilizes a thermo-conductive alloy core and highly insulative outer material to control the rate of heat removal and provide reproducible cell cryopreservation. Cell Freezing Containers are easy to use and deliver comparable results to expensive programmable freezers.

The insulative outer housing and thermo-conductive solid state core ensure consistent heat removal to all vials throughout the freezing period. Cell Freezing Containers can replace isopropanol-based freezing containers such as Mr. Frosty and others, which can consume up to 12l per year of IPA and cost hundreds of dollars per unit per year to maintain.

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  • CoolCell for Tubes & Cryo Vials
  • CoolCell for Cell Therapy Ampules
  • Key Features

CoolCell for Tubes & Cryo Vials

BCS-407P | CoolCell 1ml FX Cell Freezing Containers for 12 x 1ml 96-format Sample Tubes | With Tubes

CoolCell® 1ml FX Cell Freezing Container for 12 x 1ml 96-format Sample Tubes

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Alcohol-free CoolCell 1ml FX Cell Freezing Containers for 12 x 1ml 96-format Sample Tubes ensure controlled-rate -1℃/minute cell freezing in a -80℃ freezer

BCS-405G | CoolCell LX Cell Freezing Container for 12 x 1ml or 2ml Cryo Tubes | With Loaded Azenta Life Sciences Tubes

CoolCell® LX Cell Freezing Container for 12 x 1ml or 2ml Cryo Tubes

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Alcohol-free CoolCell LX Cell Freezing Containers for 12 x 1ml or 2ml Cryo Tubes ensure controlled-rate -1℃/minute cell freezing in a -80℃ freezer

BCS-406 | Cell Freezing Containers for 12 x 3.5ml to 5ml Cryo Tubes (formerly CoolCell 5ml LX) | With Vials

CoolCell® 5ml LX Cell Freezing Container for 12 x 3.5ml to 5ml Cryo Tubes

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Alcohol-free CoolCell 5ml LX Cell Freezing Containers for 12 x 3.5ml to 5ml Cryo Tubes ensure controlled-rate -1℃/minute cell freezing in a -80℃ freezer

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Cell Freezing Containers for 30 x 1ml or 2ml Cryo Tubes (formerly CoolCell FTS30) | Group | With Removable Cryo Tube Module (formerly CoolCell FTS30 Vial Module)

CoolCell® FTS30 Cell Freezing Container for 30 x 1ml or 2ml Cryo Tubes

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Alcohol-free CoolCell FTS30 Cell Freezing Container for 30 x 1ml or 2ml Cryo Tubes ensure controlled-rate -1℃/minute cell freezing in a -80℃ freezer

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CoolCell for Cell Therapy Ampules

BCS-172 | CoolCell® SV2 Cell Freezing Container for 12 x 2ml Injectable Cell Therapy Ampules| With Ampules

CoolCell® SV2 Cell Freezing Container for 12 x 2ml Injectable Cell Therapy Ampules

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Alcohol-free CoolCell SV2 Cell Freezing Containers for 12 x 2ml Injectable Cell Therapy Ampules ensure controlled-rate -1℃/minute cell freezing in a -80℃ freezer

BCS-262 | CoolCell SV10 Cell Freezing Container for 6 x 10ml Injectable Cell Therapy Ampules | With Ampules

CoolCell® SV10 Cell Freezing Container for 6 x 10ml Injectable Cell Therapy Ampules

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Alcohol-free CoolCell SV10 Cell Freezing Containers for 6 x 10ml Injectable Cell Therapy Ampules ensure controlled-rate -1℃/minute cell freezing in a -80℃ freezer

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Key Features

  • Safe, Secure & Competitive Cryopreservation

    Our Cell Freezing Container   Our Cell Freezing Container

    • No alcohol
      No fluids
      No pre-cooling
      Saves 12L/unit of IPA per year
    • No variability
      All vials have uniform freeze rate
      Radially symmetric design ensures vial consistency
    • No stuck lids
      Ergonomic lid comes off easily when frozen
      Not cold to the touch when removing from the -80˚C freezer
    • Quick re-use time
      Ready to use again after five minutes
    • No ongoing cost
      No alcohol purchase or disposal

    Isopropanol (IPA) Container   IPA container

    • Requires isopropanol
      Replace alcohol every 5 uses
      Track number of uses
      Pre-cool alcohol in refrigerator
    • Inconsistent freeze rate
      Alcohol degradation induces variability
      Two circles of wells; two freeze rates
    • Difficult to handle and open
      Screw cap difficult to remove when frozen
      Frozen unit is slippery and cold to touch
    • Wait between runs
      Takes >1 hour for alcohol to warmup
    • Approximately $350/year
      Change alcohol weekly
      Dispose of hazardous waste
  • Broad Cell Compatibility

    Our Cell Freezing Containers are proven to work with many cell types including:

    • Stem cells
      Human embryonic stem
      Preadipocytes
      Breast cancer stem
      Colon cancer stem
      Glioblastoma stem
      Mouse embryonic stem
      Human endothelial
      Progenitor
    • Primary cells
      Neonatal keratinocytes
      Human WBCs
      Mouse
      WBCs
      Human CD34+
      Muscle
      Human tendon
      Fibroblasts
      Melanoma tumor
      Human cardiac
      Ventricular
      Human cardiac atrial
    • Cell lines
      CHO
      LnCap
      HTB77
      A549
      HeLa
  • Cell Freezing Container Reproducibility

    Performance test: a temperature probe was placed into a 2.0 mL cryogenic vial containing 1.0 ml of water and the tube was inserted into a room temperature cell freezing container. The container was placed into a -80°C freezer and the temperature rate and profile was recorded over a 3 hour period. The test was repeated 5 consecutive times.

    Conclusion: our cell freezing container generated identical fusion time and cooling profiles over five consecutive freeze cycles.

    CoolCell® reproducibility
    Click on image to expand

    Cell freezing container reproducibility

  • Cell Freezing Container Performance vs. IPA Container

    Human embryonic stem cells
    Click on image to expand

    Human embryonic stem cells

    Human embryonic stem cells, RC-10 were frozen using the technique indicated, thawed after 2 weeks in LN2, and counted immediately (Day 1) or after 3 days of growth (Day 3).

    Alamar blue reduction assay
    Click on image to expand

    Alamar blue reduction assay

    Alamar blue reduction assay for proliferation assessment showed cells frozen in a cell freezing container grew more quickly, leading to more total cells.

     

     

    HeLA, CHO-K, K562, NIH3T3
    Click on image to expand

    HeLA, CHO-K, K562, NIH3T3

    HeLA, CHO-K, K562, NIH3T3: cell freezing container 12-well, cell freezing container FTS30 30-well or Mr. Frosty freezing containers were used to freeze all four cell lines. Identical transfection efficiencies and viabilities were observed after thawing.

    Growth performance 24 hours post thaw
    Click on image to expand

    Growth performance 24 hours post thaw

    Growth performance 24 hours post thaw: Identical growth of cells was observed 24 hours post-thaw.

  • Cell Freezing Container Performance vs. Programable Freezer

    Ag-Tregs
    Click on image to expand

    Ag-Tregs

    Ag-Tregs. Effects of freezing on antigen-specific Treg (Ag-Treg) cell therapy products; Ag-Tregs (n = 6) were frozen at concentration of 1 to 10 x 106 cells/ml using a cell freezing container or controlled rate freezer (CRF) with a freezing rate of -1°C/min. Viability and absolute viable cell count of thawed Ag-Treg cell therapy products were evaluated by flow cytometry.

    - Data generated by TxCell SA

    Comparison of freezing methods
    Click on image to expand

    Comparison of freezing methods

    Comparison of freezing methods. Graph comparing % of cell recovery after freezing with the cell freezing container (blue) versus freezing using a programmable step-down freezer (red) in 3 different samples at high cell concentration. There was no significant difference between the two freezing methods.

    - Data performed by UCSF Diabetes Center

  • Cell Freezing Container Protocols

    Cryopreservation and Thawing of Cells

    Cryopreservation and Thawing of Cells

    Wayne M. Yokoyama, Maria L. Thompson, Rolf O. Ehrhardt
    University of California School of Medicine, San Francisco, CA
    BioCision LLC, Larkspur, California
    Curr. Protoc. Immunology. 2012 Nov; 99 Appendix 3G

     

    Standardized Cryopreservation of Pluripotent Stem Cells

    Standardized Cryopreservation of Pluripotent Stem Cells

    Rick I. Cohen, Maria L. Thompson, Brian Schryver, Rolf O. Ehrhardt
    Rutgers University, Piscataway, New Jersey
    BioCision LLC, San Rafael, California
    Curr. Protoc. Stem Cell Biol. 28:1C.14.1-1C.14.10

     

    Standardized Cryopreservation of Human Primary Cells

    Standardized Cryopreservation of Human Primary Cells

    Thomas V. Ramos, Aby J. Mathew, Maria L. Thompson, Rolf O. Ehrhardt
    HemaCare Corporation, Van Nuys, California, BioLife Solutions, Bothell, Washington, BioCision, Larkspur, California
    Curr. Protoc. Cell Biology. 2014 Sept; 64 Appendix 3I.

 

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