CoolCell® Cell Freezing Containers

Alcohol-Free Cell Freezing Containers: Standardized, Passive Controlled Rate Freezing

CoolCell Alcohol-Free Cell Freezing Containers ensure standardized controlled-rate -1˚C/minute cell freezing in a -80˚C freezer - without alcohol or any fluids. Proven for use with a variety of cell types including stem cells, primary cells, PBMC cell lines, insect cells, yeast and others.

The patent-pending CoolCell Alcohol-Free Cell Freezing Container technology utilizes a thermo-conductive alloy core and highly insulative outer material to control the rate of heat removal and provide reproducible cell cryopreservation. Cell Freezing Containers are easy to use and deliver comparable results to expensive programmable freezers.

The insulative outer housing and thermo-conductive solid state core ensure consistent heat removal to all vials throughout the freezing period. Cell Freezing Containers can replace isopropanol-based freezing containers such as Mr. Frosty and others, which can consume up to 12l per year of IPA and cost hundreds of dollars per unit per year to maintain.

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CoolCell for Tubes & Cryo Vials
CoolCell for Cell Therapy Ampules
Key Features

CoolCell for Tubes & Cryo Vials

BCS-407P | CoolCell 1ml FX Cell Freezing Containers for 12 x 1ml 96-format Sample Tubes | With Tubes

CoolCell® 1ml FX Cell Freezing Container for 12 x 1ml 96-format Sample Tubes

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Alcohol-free CoolCell 1ml FX Cell Freezing Containers for 12 x 1ml 96-format Sample Tubes ensure controlled-rate -1℃/minute cell freezing in a -80℃ freezer

CoolCell® LX Cell Freezing Container for 12 x 1ml or 2ml Cryo Tubes

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Alcohol-free CoolCell LX Cell Freezing Containers for 12 x 1ml or 2ml Cryo Tubes ensure controlled-rate -1℃/minute cell freezing in a -80℃ freezer

BCS-406 | Cell Freezing Containers for 12 x 3.5ml to 5ml Cryo Tubes (formerly CoolCell 5ml LX) | With Vials

CoolCell® 5ml LX Cell Freezing Container for 12 x 3.5ml to 5ml Cryo Tubes

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Alcohol-free CoolCell 5ml LX Cell Freezing Containers for 12 x 3.5ml to 5ml Cryo Tubes ensure controlled-rate -1℃/minute cell freezing in a -80℃ freezer

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Cell Freezing Containers for 30 x 1ml or 2ml Cryo Tubes (formerly CoolCell FTS30) | Group | With Removable Cryo Tube Module (formerly CoolCell FTS30 Vial Module)

CoolCell® FTS30 Cell Freezing Container for 30 x 1ml or 2ml Cryo Tubes

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Alcohol-free CoolCell FTS30 Cell Freezing Container for 30 x 1ml or 2ml Cryo Tubes ensure controlled-rate -1℃/minute cell freezing in a -80℃ freezer

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CoolCell for Cell Therapy Ampules

CoolCell® SV2 Cell Freezing Container for 12 x 2ml Injectable Cell Therapy Ampules

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Alcohol-free CoolCell SV2 Cell Freezing Containers for 12 x 2ml Injectable Cell Therapy Ampules ensure controlled-rate -1℃/minute cell freezing in a -80℃ freezer

CoolCell® SV10 Cell Freezing Container for 6 x 10ml Injectable Cell Therapy Ampules

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Alcohol-free CoolCell SV10 Cell Freezing Containers for 6 x 10ml Injectable Cell Therapy Ampules ensure controlled-rate -1℃/minute cell freezing in a -80℃ freezer

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Key Features

Safe, Secure & Competitive Cryopreservation
Our Cell Freezing Container
- No alcohol
  No fluids
  No pre-cooling
  Saves 12L/unit of IPA per year
- No variability
  All vials have uniform freeze rate
  Radially symmetric design ensures vial consistency
- No stuck lids
  Ergonomic lid comes off easily when frozen
  Not cold to the touch when removing from the -80˚C freezer
- Quick re-use time
  Ready to use again after five minutes
- No ongoing cost
  No alcohol purchase or disposal
Isopropanol (IPA) Container
- Requires isopropanol
  Replace alcohol every 5 uses
  Track number of uses
  Pre-cool alcohol in refrigerator
- Inconsistent freeze rate
  Alcohol degradation induces variability
  Two circles of wells; two freeze rates
- Difficult to handle and open
  Screw cap difficult to remove when frozen
  Frozen unit is slippery and cold to touch
- Wait between runs
  Takes >1 hour for alcohol to warmup
- Approximately $350/year
  Change alcohol weekly
  Dispose of hazardous waste
Broad Cell Compatibility
Our Cell Freezing Containers are proven to work with many cell types including:
- Stem cells
  Human embryonic stem
  Preadipocytes
  Breast cancer stem
  Colon cancer stem
  Glioblastoma stem
  Mouse embryonic stem
  Human endothelial
  Progenitor
- Primary cells
  Neonatal keratinocytes
  Human WBCs
  Mouse
  WBCs
  Human CD34+
  Muscle
  Human tendon
  Fibroblasts
  Melanoma tumor
  Human cardiac
  Ventricular
  Human cardiac atrial
- Cell lines
  CHO
  LnCap
  HTB77
  A549
  HeLa
Cell Freezing Container Reproducibility

Performance test: a temperature probe was placed into a 2.0 mL cryogenic vial containing 1.0 ml of water and the tube was inserted into a room temperature cell freezing container. The container was placed into a -80°C freezer and the temperature rate and profile was recorded over a 3 hour period. The test was repeated 5 consecutive times.

Conclusion: our cell freezing container generated identical fusion time and cooling profiles over five consecutive freeze cycles.

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Cell freezing container reproducibility
Cell Freezing Container Performance vs. IPA Container

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Human embryonic stem cells

Human embryonic stem cells, RC-10 were frozen using the technique indicated, thawed after 2 weeks in LN2, and counted immediately (Day 1) or after 3 days of growth (Day 3).

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Alamar blue reduction assay

Alamar blue reduction assay for proliferation assessment showed cells frozen in a cell freezing container grew more quickly, leading to more total cells.

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HeLA, CHO-K, K562, NIH3T3

HeLA, CHO-K, K562, NIH3T3: cell freezing container 12-well, cell freezing container FTS30 30-well or Mr. Frosty freezing containers were used to freeze all four cell lines. Identical transfection efficiencies and viabilities were observed after thawing.

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Growth performance 24 hours post thaw

Growth performance 24 hours post thaw: Identical growth of cells was observed 24 hours post-thaw.
Cell Freezing Container Performance vs. Programable Freezer

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Ag-Tregs

Ag-Tregs. Effects of freezing on antigen-specific Treg (Ag-Treg) cell therapy products; Ag-Tregs (n = 6) were frozen at concentration of 1 to 10 x 106 cells/ml using a cell freezing container or controlled rate freezer (CRF) with a freezing rate of -1°C/min. Viability and absolute viable cell count of thawed Ag-Treg cell therapy products were evaluated by flow cytometry.

- Data generated by TxCell SA

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Comparison of freezing methods

Comparison of freezing methods. Graph comparing % of cell recovery after freezing with the cell freezing container (blue) versus freezing using a programmable step-down freezer (red) in 3 different samples at high cell concentration. There was no significant difference between the two freezing methods.

- Data performed by UCSF Diabetes Center
Cell Freezing Container Protocols

Cryopreservation and Thawing of Cells

Wayne M. Yokoyama, Maria L. Thompson, Rolf O. Ehrhardt
University of California School of Medicine, San Francisco, CA
BioCision LLC, Larkspur, California
Curr. Protoc. Immunology. 2012 Nov; 99 Appendix 3G

Standardized Cryopreservation of Pluripotent Stem Cells

Rick I. Cohen, Maria L. Thompson, Brian Schryver, Rolf O. Ehrhardt
Rutgers University, Piscataway, New Jersey
BioCision LLC, San Rafael, California
Curr. Protoc. Stem Cell Biol. 28:1C.14.1-1C.14.10

Standardized Cryopreservation of Human Primary Cells

Thomas V. Ramos, Aby J. Mathew, Maria L. Thompson, Rolf O. Ehrhardt
HemaCare Corporation, Van Nuys, California, BioLife Solutions, Bothell, Washington, BioCision, Larkspur, California
Curr. Protoc. Cell Biology. 2014 Sept; 64 Appendix 3I.