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This whitepaper will help you understand the ideal fill amount for your samples, and how controlled-rate freezing can positively impact storage of those samples.
Exposure of cryopreserved biological material to ambient temperatures may reduce the functionality and viability of the stored material, particularly when sample temperature
It seems to be universally accepted that storing at -190°C in a liquid nitrogen (LN2) vapor-phase freezer is the best technique for biosample preservation.