Preserve long poly(A) tails for optimal mRNA plasmid stability
Due to their versatility for protein expression, synthetic mRNA plasmids have become an ideal tool for gene therapies. A key component of an mRNA plasmid is the poly(A) tail, which determines the structural integrity of the molecule. While long poly(A) sequences have generally resulted in increased stability of mRNA products, poly(A) tails with greater than 100 bases can be susceptible to truncations during production, resulting in unstable mRNA products.
To address this challenge, Azenta Life Sciences has developed an efficient protocol using a proprietary E. coli strain and optimized culturing methods to ensure long poly(A) sequence stability of at least 140 bases in mRNA plasmids. In this tech note, discover how this method can help you achieve high-fidelity templates for in vitro transcription (IVT).
What you’ll learn:
- How synthetic mRNA plasmids are developed for IVT
- Benefits and challenges of synthetic mRNA plasmid preparation
- Comparison of standard preparation protocols and the proprietary protocol from Azenta
Complete the form to learn how to achieve IVT templates of higher quality and yield.
